实时荧光定量PCR检测食管癌循环miRNA的研究  

Real-time PCR in detection of plasmic miRNA in patients with esophageal cancer

作　　者：彭学宏 陈暖[2] 李克[3] 郑邦希 

机构地区：[1]广东省汕头市潮南民生医院,515000 [2]汕头大学第一附属医院 [3]汕头大学医学院

出　　处：《中国实用医刊》2014年第6期1-2,共2页Chinese Journal of Practical Medicine

基　　金：广东省科技计划项目资助（20101096）

摘　　要：目的研究miR-424、miR-373、miR-34c、miR-129、miR-130b、miR-203、miR-9、miR-15b、miR-100、miR-145、miR-21在食管癌患者外周血的差异表达。方法采用Real-timePCR定量检测方法分析比较20例食管癌患者血液与健康对照者血液之间miR-424、miR-373、miR-34c、miR-129、miR-130b、miR-203、miR-9、miR-15b、miR-100、miR-145、miR-21表达差异情况。结果miR-424、miR-373、miR-34c、miR-203、miR-15b表达上调，miR-100表达下调。结论外周血miRNA可能成为食管癌患者早期诊断新的生物学指标。Objective To investigate the differential expression of miR-424, miR-373, miR-34c, miR-129, miR-130b, miR-203, miR-9, miR-15b, miR-100, miR-145, miR-21 in serum of esophageal cancer. Methods The expression levels of miR-424, miR-373, miR-34c, miR-129, miR-130b, miR-203, miR-9, miR-15b, miR-100, miR-145, miR-21 were detected by real-time PCR in serum of 20 human esopha- geal cancer and 20 normal person. Results Over-expression of miR-424, miR-373, miR-34c, miR-203, miR-15b and low-expression of miR-100 were observed in serum of esophageal cancer. Conclusions miRNAs in the serum maybe a new biological indicator for diagnosis of human esophageal cancer.

关 键 词：食管癌 实时荧光定量PCR MICRORNAS 

分 类 号：R735.1[医药卫生—肿瘤]