胃癌组织中靶向Abl相互作用蛋白-1的microRNA筛选  

Screening microRNA of targeting Abelson interactor protein-1 in gastric carcinoma

作　　者：崔梅花[1,2] 郁卫东[3] 李晓宇[1,2] 张秀茹[4] 傅怡[1,2] 岳林[1,2] 

机构地区：[1]航天中心医院 [2]北京大学航天临床医学院消化科,北京100049 [3]北京大学人民医院临床分子生物学研究所,北京100044 [4]航天中心医院病理科,北京100049

出　　处：《中国现代医学杂志》2013年第36期39-42,共4页China Journal of Modern Medicine

基　　金：首都医学发展科研基金(No:2009-3174);航天中心医院科研基金(No:200904)

摘　　要：目的检测3种Abl相互作用蛋-1(ABI-1)相关的microRNA(miRNA)在胃癌组织及癌旁正常组织中的表达模式,筛选出与胃癌组织相关的靶向ABI-1的miRNA。方法收集59例胃癌术后蜡块标本,包含胃癌组织和癌旁正常组织,抽提石蜡组织中的总RNA。在基因网选择ABI-1相关的miRNA(即miR-181c、miR-148b、miR-9),采用逆转录实时荧光定量PCR法对胃癌组织及癌旁正常组织中的3种miRNA进行检测,计算胃癌组织相对癌旁正常组织中miRNA的相对表达量(2-ΔΔCt)。结果 miR-181c、miR-148b、miR-9在胃癌组织中的相对表达量分别为(2.32±1.04)(P=0.000)、(0.82±0.67)(P=0.321)、(1.07±0.95)(P=0.774),其中miR-181c在胃癌组织及癌旁正常组织中的表达差异有显著性(P=0.000)。结论 miR-181c在胃癌组织中相对高表达,提示其可能作为靶向ABI-1的miRNA调节ABI-1蛋白的表达,为进步研究靶向ABI-1miRNA在胃癌发生发展中的作用奠定基础。[ Objective ] To detect the expression patterns of three kinds of microRNA （miRNA） related to Abelson interactor protein-/ （ABI-I） in gastric carcinoma and adjacent normal tissue, and screen miRNA targeting ABI-1 which may be relevant to gastric carcinoma. [ Methods ] Tissue specimens were from 59 gastric cancer patients who underwent gastrectomy in our hospital, which were nlatched gastric carcinoma and adjacent normal tissue samples. Total RNA in paraffin-embedded tissues were extracted, miRNA （miR-181c, miR-148b, miR-9） relevant to ABI-1 were selected from Gene network. The expression of three miRNAs in gastric carcinoma and adjacent normal tissue were measured using reverse transcriptase real-time quantitative PCR, and the relative expression level （2-am） in gastric cancer tissues were calculated. [ Results ] The relative expression levels of miR-181c, miR-148b and miR-9 in gastric cancer tissues were （2.32±1.04）（P =0.000）, （0.82±0.67）（P =0.321）, （1.07±0.95）（P =0.774）, respectively. The difterenee of expression of miR-181c in gastric carcinoma and adjacent normal tissue was statistically significant （P =0.000）. [Conclusions] The relatively high miR-181c expression in gastric cancer tissues suggests that miR-181c maybe target ABI-1 and regulate the ABI-1 protein expression. These results lay the foundation for further study of the role of miRNA targeting ABI-1 in the pathogenesis of gastric cancer.

关 键 词：microRNA(miRNA) miR-181c Abl相互作用蛋白-1(ABI-1) 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]