灯盏花素通过抑制MCP-1的表达对L-精氨酸诱发重症急性胰腺炎小鼠的治疗作用研究  

Effect of Breviscapine inhibiting MCP-1 on severe acute pancreatitis induced by L-Arginine in mice

作　　者：张晓芹[1] 贾晓云[2] 史迎莉[1] 马晓军[1] 李涛[1] 张红[1] 

机构地区：[1]陕西中医学院病理生理学教研室,陕西咸阳712046 [2]吉林省敦化市医院病理科,吉林敦化133700

出　　处：《中国现代医学杂志》2013年第35期17-21,共5页China Journal of Modern Medicine

基　　金：陕西省自然科学研究基础资助项目(No:2010JM4023)

摘　　要：目的观察L-精氨酸诱发的重症急性胰腺炎小鼠胰腺组织MCP-1 mRNA表达的变化,以及灯盏花素对MCP-1 mRNA表达的影响,从而探讨MCP-1在急性胰腺炎中的作用和灯盏花素治疗急性胰腺炎的机制。方法健康雄性成年昆明种小鼠30只,随机分为3组(n=10):对照组(Control)、模型组(SAP)和灯盏花素组(SAP+Breviscapine)。除对照组外,其余各组给予腹腔注射20%L-精氨酸(3 g/kg×2次,间隔1 h);灯盏花素组在第2次腹腔注射20%L-精氨酸30 min后给予腹腔注射灯盏花素(100 mg/kg),之后每天注射2次。在造模后72 h麻醉处死动物检测血清淀粉酶活性;取胰腺组织计算胰腺湿重比,HE染色观察胰腺病理学改变;取肺组织匀浆检测髓过氧化物酶(MPO)的活性,HE染色观察肺病理学改变;Real-time PCR检测胰腺MCP-1 mRNA的表达变化。结果 L-精氨酸诱发急性胰腺炎72 h后,血清淀粉酶活性明显升高,胰腺湿重比增加,肺组织MPO显著增加,与对照组比较差异有统计学意义(P<0.05);而灯盏花素组血清淀粉酶的活性、胰腺湿重比、MPO水平明显降低,与模型组相比差异具有统计学意义(P<0.01);造模组72 h胰腺及肺可见明显病理损伤,胰腺组织MCP-1 mRNA的表达明显增强;灯盏花素组胰腺及肺病理损伤减轻,胰腺MCP-1mRNA的表达减弱。结论 L-精氨酸诱发的重症急性胰腺炎小鼠胰腺组织MCP-1 mRNA表达明显增强,MCP-1参与了L-精氨酸诱发的急性胰腺炎进展;灯盏花素治疗急性胰腺炎的作用机制之一与抑制炎症反应有关。【Objective】 To explore the role of MCP-1 in acute pancreatitis induced by L-Arginine,and the mechanisms of Breviscapine treating on severe acute pancreatitis（SAP）. 【Methods】 The KM mice were randomly divided into 3 groups（n =10）： control group; SAP group（L-Arginine, L-arg）; Breviscapine group（SAP ＋ Breviscapine）. Except for Control group, the mice in other groups were injected with 20% L-Arginine（3 g/kg, twice） intraperitoneally. The mice in Breviscapine group were injected intraperitoneally with Breviscap- ine（100 mg/kg） after 30 min of the second injection of 20% L-Arginine and twice a day at the following two days. At 72 h, the mice were anesthetized and sacrificed. The activity of amylase was measured in serum, the relative pancreatic weight was assayed, and the myeloperoxidase（MPO） activity was analyzed to evaluate the neutrophil infiltration in lung tissue. The morphology of pancreas and lung was observed. The RNA of pan- creas was extracted to detect the expression of MCP-1 mRNA by Real-time PCR. 【Results】 At 72 h, LArginine induced SAP with increased serum amylase activity, pancreatic wet weight ratio and MPO activity in lung tissue（P 0.05）. While Breviscapine group, the activity of amylase, pancreatic wet weight ratio and MPO levels were significantly lower. Compared with the SAP group, the difference was significant（P 0.01）; At 72 h, the pancreas and lung were obvious injury. The expression of MCP-1 mRNA in pancreas tissue increased significantly. In Breviscapine group, the pathologic damage of pancreas and lung tissue, MCP-1 mRNA expres- sion in pancreas were reduced significantly. 【Conclusion】 The expression of MCP-1 mRNA in pancreas in- creased in the mice with severe acute pancreatitis induced by L-Arginine. The activation of MCP-1 maybe takes part in the development of SAP. To inhibit the activation of MCP-1 in pancreas is the one of mecha- nisms that Breviscapine treats SAP.

关 键 词：单核趋化蛋白-1 急性胰腺炎 灯盏花素 

分 类 号：R363[医药卫生—病理学] R657.51[医药卫生—基础医学]