Hart改良法在判断有否胃癌脉管侵袭中的应用  

Application of Hart improved method in determination of vessel invasion in gastric carcinoma

作　　者：钟志玖[1] 金晓明[1] 孙宇[1] 毕彦忠[1] 

机构地区：[1]哈尔滨医科大学病理学教研室,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2001年第1期17-18,24,F003,共4页Journal of Harbin Medical University

基　　金：归国留学基金资助项目!( 980 0 679);黑龙江省自然科学基金资助项目!(D9847)

摘　　要：目的　探讨Hart改良的弹力纤维染色法在判断胃癌脉管侵袭中的应用价值。方法　对 6 6例石蜡包埋胃癌组织分别进行HE染色 ,Hart改良的弹力纤维染色 ,光镜下观察 6 6例胃癌的组织学分类、淋巴管和脉管侵入情况。结果　 6 6例胃癌中有血管侵入者 5 0例 (74% ) ,有粘膜下淋巴管侵入者 6 0例 (91% ) ,在癌组织侵袭的淋巴管周围 ,血管内有癌细胞侵入者 47例 (78% )。结论　Hart改良的弹力纤维染色法可使一些较细的弹力纤维着色 ,效果强于其它弹力纤维染色法 ,特异性与免疫组化相比差别不大 ,易操作且经济 ,应当考虑将其作为肿瘤诊断中的一个常规染色法加以应用。Objective To study the practical value of Hart improved elastic fiberstain(HIEFS) in determination of vessel invasion in gastric carcinoma. Methods 66 cases of gastric carcinoma tissues were stained with Hematoxylin-eosin staining and HIEFS. Histological classification, lymphatics and vessel invasion were observed by light microscope.Results Among 66 cases of gastric carcinoma tissues, vessel invasion were observed in 50(74%) cases, lymphatics invasion were found in 60 (91%) cases, in which 47 (78%) cases were accompanied with vessel invasion.Conclusion We can get a better result by HIEFS than other elastic fiber stain methods, for it can dye those tiny elastic fiber. No significant difference between HIEFS and IHC. HIEFS is not only easy to operate, but also economic. It should be considered to make it a routine stain method in the work of tumor diagnosis.

关 键 词：胃肿瘤 血管侵袭 弹力纤维染色法 HArt改良法 

分 类 号：R735.2[医药卫生—肿瘤] R73－37[医药卫生—临床医学]