2009年云南省柯萨奇病毒B5：分离株A210／KM／09全基因序列分析  

Genomic characteristics of coxsackievirus B5 A210/KM/09 strain isolated in Yunnan, China

作　　者：刘建生[1,2] 邵聪文[1,2] 赵卫中[1,2] 张云昆[1,2] 吉玛[1,2] 朱艳菊[1,2] 马忠飞[1,2] 马绍辉[1,2] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,昆明650118 [2]云南省重大传染病疫苗研发重点实验室

出　　处：《中华流行病学杂志》2014年第3期307-311,共5页Chinese Journal of Epidemiology

基　　金：云南省应用基础研究项目（2013FZl36）

摘　　要：目的分析柯萨奇病毒B5（CVB5）云南分离株A210／K2~I／09全基因序列及其遗传特性。方法设计针对CVB5引物，提取病毒RNA，RT-PCR扩增和产物直接测序获得序列，利用Mega4．1、RDP3和SimPlot3．5．1软件分析全基因序列。结果CVB5A210／KM／09全基因组核苷酸序列长度为7372bp，编码含2185个氨基酸残基的多聚蛋白；A210／KM／09全基因组核苷酸及所推导的氨基酸与CVB5／CCl0／10同源性最高，其同源性分别为92．5％和97.3％；而与其他CVB5毒株如17Y、19CSF、20CSF、1954／85／US、2000／CSF／KOR、03001N、CoxB5／Henard2010、CVB5／SD／09和Faulkner核苷酸和氨基酸同源性分别为80．1％～92．5％和95．0％～97.3％；A210／KM／09与其他CVB5毒株的各区段核苷酸和氨基酸同源性分别为75.3％～96.3％和85．3％～100．O％，其中与VP3、3D区段核苷酸的同源性最低。基于CVB5全长VPl基因序：列进行种系进化分析，可将CVB5分为A、B、C和D4个基因型，其中C基因型可再分为C1～C4基因亚型，D基因型可再分为D1～D4基因亚型。中国CVB5流行株主要聚集在C4基因亚型，国外流行株主要聚集在D1、C2亚型。结论A210／KM／09分离株为C4基因型。Objective To characterize the complete genome sequence of coxsackievirus B5 （CVB5） A210/KM/09 strain which was isolated fi＇om Yunnan, China, 2009. Methods Eight overlapping clones covering the whole viral genome （excluding the poly-A tail） were obtained by RT-PCR and sequenced, with their nucleotide and amino acid sequences compared with other known CVB5 strains. Results The genome of the CVB5 A210/KM/09 strain had 7 372 nucleotides in length, and containing a 742-nt non-translated region （NTR） at the 5＇ end and a 98-nt NTR at the 3＇ end. The entire open reading frame contained 6 555 nt, encoding a 2 185-aa polyprotein. In the coding region, there appeared no nucleotide deletion or insertion, but some changes of amino acid seemed unique. Based on the complete genome sequence alignments, CVB5 isolate A210/KM/09 strain showed the highest nucleotide （92.5%） and amino acid （97.3%） identities to the CVB5/CCIO/IO. It also shared nucleotide （ 80.1%-92.5 % ） and amino acid （95.0%-97.3 % ） homology ＇with other CVB5 strains： 17Y, 19CSF, 20CSF, 1954/85/US, 2000/CSF/KOR, 03001N, CoxB5/Henan/2010, VB5/SD/09 and Faulkner. Blast between genome fragments, A210/KM/09 showed similarity on nucleotide （80.1%-92.5%） and amino acid （95.0%-97.3%） identities with other CVB5 strains. The phylogenetic tree, constructed on the complete VP 1 regions, indicated that CVB5 could be divided into genotype A, B, C and D. while Genotype C and D could be further divided into C1--C4 and DI-D4 subgenotypes. Conclusion A210/KM/09 and other CVB5 predominant strains isolated in China belonged to CVB5 subgenotype C4.

关 键 词：柯萨奇B组5型病毒 全基因组 序列分析 进化树图 

分 类 号：R373[医药卫生—病原生物学]