STR-PCR定量检测供受者嵌合体方法的建立及临床应用  

QUANTITATIVE ASSESSMENT OF MIXED HEMATOPOIETIC CHIMERISM BY STR-PCR AFTER NONMYLOABLATIVE ALLOGENEIC STEM CELL TRANSPLANTATION

作　　者：孙琪云[1] 艾辉胜[1] 姚波[1] 余长林[1] 郭梅[1] 吕星[2] 路萍[2] 

机构地区：[1]军事医学科学院附属医院,北京100039 [2]军事医学科学院放射医学研究所

出　　处：《解放军医学杂志》2001年第1期13-15,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：利用STRs的高度多态性等特性 ,用竞争性聚合酶链反应 (PCR)结合聚丙烯凝胶电泳、染色和凝胶成像分析等技术 ,建立定量检测异基因造血干细胞移植后供受者嵌合体的方法 ,用无关个体的DNA混合实验进行可行性和准确性研究 ,并同时做染色体分带进行对照。在此基础上对 2例非清髓异基因造血干细胞移植病人的植入情况进行动态检测。结果显示 ,扩增产物中供受者DNA含量的百分比同扩增前混合样本的比例呈显著直线相关 ,STR PCR检测嵌合体方法简单、快速、可靠、敏感性高 ,所需标本量少且不受性别限制 ,是检测NAST供受者嵌合体的新方法。To establish a method for quantitative assessment of mixed hematopoietic chimerism by competitive polymerase chain reaction after nonmyeloablative allogeneic stem cell transplantation(NAST) using highly polymorphic short tandem repeats (STRs). The feasibility of this assay and the accuracy of quantitative results were tested using serial DNA mixtures of unrelated individuals. 2patients receiving NAST were analyzed by this method. Chromosomewas analyzed simultaneously. The results showed a linear correlation between the DNA added and the proportion found. Comparison of values obtained with chromosomeanalysis showed an excellent correlation with the STR PCR results. Taken together, this novel approach allows a rapid, sensitive, versatile, less blood , even with minuscule numbers of cells,and also sex unlimited.

关 键 词：嵌合体 异基因造血干细胞移植 短串联重复序列 STR-PCR 白血病 

分 类 号：R733.7[医药卫生—肿瘤] R457[医药卫生—临床医学]