p38信号途径与胃癌细胞阿霉素耐药相关  

THE EFFECTS OF ADRIAMYCIN ON THE ACTIVITY OF p38 MAPK IN GASTRIC CANCER CELL SGC7901 AND MULTIDRUG RESISTANT CELL SGC7901/VCR

作　　者：王雨田[1] 胡家露[2] 药立波[3] 王吉村[3] 张忠兵[2] 樊代明[2] 王少东[2] 

机构地区：[1]第二军医大学长征医院,上海200003 [2]西安第四军医大学西京医院消化内科 [3]第四军医大学生化教研室

出　　处：《解放军医学杂志》2001年第1期24-26,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金!(编号 39470 785);全军医药卫生青年基金!(编号 96Q0 78)

摘　　要：研究 p38MAPK信号转导途径在胃癌耐药机制中的意义。用免疫共沉淀法及Westernboltting实验分别研究阿霉素处理胃癌细胞SGC790 1及胃癌多药耐药SGC790 1/VCR细胞后 ,p38激酶活性及量的变化。结果显示 ,阿霉素处理胃癌多药耐药SGC790 1/VCR细胞 15min后 ,p38MAPK活性明显降低 ,且发现 p38MAPK量也明显下降。与之不同 ,阿霉素处理SGC790 1细胞后 ,p38MAPK活性增强 ,呈时间依赖性 ,而 p38MAPK量无明显变化。提示肿瘤化疗药物阿霉素可诱导肿瘤耐药细胞 p38激酶活性降低 ,且可诱导非耐药细胞p38激酶活性增强。To investigatethe relationship between p38 MAPK pathway and multidrug resistance of stomach cancer. The activity of p38 MAP kinase in stomach cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR were examined by immunoprecipition after adriamycin treatment SGC7901 for 0, 2, 15, 30 and SGC7901/VCR for 0, 2, 15, 30, 60 minutes,respectively. The results indicated that adriamycin could activate the activity of p38 MAP kinase in gastric cancer cell SGC7901 with time dependency, but could decrease remarkably the activity of p38 MAP kinase in gastric cancer multidrug resistant cell SGC7901/VCR after adriamycin treatment it for 15 minutes. It suggested that adriamycin could affect the activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR.

关 键 词：P38MAPK 信号转导 胃癌 多药耐药性 阿霉素 免疫共沉淀法 

分 类 号：R735.2[医药卫生—肿瘤]