Fgβ-148 C/T基因多态性对缺血性脑卒中患者血浆Fg水平的影响  

Fibrinogenβ-148 gene polymorphism associated with plasma fibrinogen levels in ischemic stroke

作　　者：杨志刚[1] 刘国勋[1] 李凤芹[1] 凌光鑫[1] 程爽[1] 蔡望伟[2] 

机构地区：[1]广东医学院附属医院血液病研究室,广东湛江524001 [2]海南医学院生化教研室

出　　处：《中国实验诊断学》2001年第1期12-14,共3页Chinese Journal of Laboratory Diagnosis

基　　金：卫生部课题基金资助项目!(98-1-348)

摘　　要：目的　分析纤维蛋白原（Ｆｇ）β－１４８ Ｃ／Ｔ基因多态性与性别、年龄、吸烟等因素对血浆Ｆｇ水平的影响。方法用ＰＣＲ－ＲＦＬＰ的方法分析基因多态性，比浊法测定血浆Ｆｇ水平。结果病例组血浆Ｆｇ７ｋ平明显高于对照组（Ｐ＜．０１），两组的Ｔ－１４８基因频率分布差异无显著性（Ｐ＞０．０５）。男女两性的血浆Ｆｇ水平比较差异无显著性（Ｐ＞０．０５）：两性中Ｔ－１４８基因携带者血浆Ｆｇ水平均比 ＣＣ基因型者明显升高（Ｐ＜０．０５）。在 Ｔ－１４８携带者中，血浆 Ｆｇ 水平随年龄增长而升高 （Ｐ＜０．０５）；吸烟组 Ｆｇ 水平高于不吸烟与戒烟组（Ｐ＜０．０５）。结论 Ｆｇβ－１４８ Ｃ／Ｔ基因多态性与血浆 Ｆｇ水平相关，Ｔ－１４８基因携带者更易受年龄、吸烟等因素影响使血浆Ｆｇ水平升高。提示Ｆｇβ－１４８ Ｃ／Ｔ基因多态性可作为血栓性疾病的遗传易感标志之一。Objective To analyze the associations of β-fibrinogen gene-148 C/T polymorphism, gender, age and smoking habit with plasma fibrinogen levels. Methods The Fgβ -148 C/R gene polymorphism was analyzed by PCR-RFLP. And plasma fibrinogen levels were determined by Turbimetry,Results Cases was similar in frequency of T-148 allele to con- trol subjects (P>0.05). No significant difference in frequency of T-148 allele was seen in experiment and control pops (P< 0.05).The plasma fibrinogen levels in patients were significantly higher than those in the control subjects(P<0.01). There were no significant differences in plasma fibrinogen levels between men and women (P>0.05); those with T-148 alleles had significant higher plasma fibrinogen levels than those without T-148 alleles(P<0.05). In those with T-148 allele, the Plasma fibrinogen levels were significantly different between the different age groups(P<0.05); smokers had significantly higher plasma fibrinogen levels than non-smokers and ex-smokers (P<0.05). Conclusion Fgβ -148 C/T substitution is a strong and consistent predictor of plasma fibrinogen levels, but the degree of the association is affected by age and smoking, and the T-148 allele may be a susceptible predictor of ischemic stroke.

关 键 词：纤维蛋白原 基因多态性 吸烟 年龄 脑缺血 Fgβ-148C/T FG 

分 类 号：R743[医药卫生—神经病学与精神病学]