高强度聚焦超声制备小鼠(H_(22))肝癌固化瘤苗免疫性的实验研究  

Experimental Study on the Immunity of Solidified H_(22) Liver Tumor Vaccine in Mice Treated by HIFU

作　　者：付敏[1] 赖春冬[1] 周颦[1] 杜永洪[2] 邹建中[2] 伍烽[2] 白晋[2] 王智彪[2] 

机构地区：[1]重庆市第一人民医院肿瘤科,400011 [2]重庆医科大学医学超声工程研究所

出　　处：《临床超声医学杂志》2001年第1期1-3,共3页Journal of Clinical Ultrasound in Medicine

基　　金：国家自然科学基金!(39630 34)

摘　　要：目的　对比研究经高强度聚焦超声 (HIFU)及经 6 5℃高温水浴 1小时处理后制成的固化瘤苗的细胞免疫效应。方法　将小鼠H2 2 肝癌细胞分别用HIFU及 6 5℃高温水浴 1小时处理 ,制成固化瘤苗 ,并将两种方法制得的瘤苗同时接种于不同的小鼠 ,以抵抗同源H2 2 癌细胞 1.0× 10 7/ml攻击 ,比较其抑瘤率和 6周内生命延长率。结果　经HI FU处理制成的瘤苗其抑瘤率和 6周内生命延长率均显著高于经高温水浴法制得者 (P <0 .0 0 5 )。结论　HIFU瘤苗组具有明显优于高温瘤苗组的细胞免疫效应 。Objective To comparatively study the immunity of H 22 liver tumor vaccines of mice treated by high-intensity focused ultrasound (HIFU) and heating for one hour in 65℃ water.Methods H 22 liverrtumor cells of mice seperately treated by HIFU and heating for one hour in 65℃ water to acquire the vaccines of liver tumor cells.The vaccines obtained from the two methods were vaccinated to different mice to antagonize the attact of 1.0×10 7/ml H 22 tumor cells.We observed the survive rate and life prolonging rate of those mice in 6 weeks after vaccination.Result The survive rate and life prolonging rate of those mice treated by HIFU were higher than that of those treated by heating (P<0.005).Conclusion The immune effect of H 22 liver tumor vaccine made by HIFU is superior to that made by heating.HIFU is a good way to make tumor vaccine.

关 键 词：高强度聚焦超声 肝癌 瘤苗 细胞免疫 免疫治疗 

分 类 号：R735.7[医药卫生—肿瘤] R73－36[医药卫生—临床医学]