苋菜试管苗amaNACA 2-like基因克隆及生物信息学分析  

Cloning and bioinformatics analysis of amaNACA 2-like from in vitro plantlets in Amaranthus tricolor L.

作　　者：刘生财[1] 匡华琴[1] 谢礼洋[1] 赖钟雄[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002

出　　处：《东北农业大学学报》2014年第1期83-89,共7页Journal of Northeast Agricultural University

基　　金：高等学校博士学科点专项新教师类科研基金项目(20123515120009);福建省自然科学基金项目(2013J05045);福建省教育厅A类项目(JA12098);福建农林大学园艺学博士后基金项目(132300102);福建农林大学园艺学院青年学术骨干培养基金项目(FAFU2012 YYPY10)

摘　　要：以苋菜试管苗为材料,采用RT-PCR结合RACE技术进行amaNACA-2 like基因全长cDNA序列克隆,对其进行生物信息学分析。结果表明,苋菜amaNACA-2 like基因cDNA全长为896 bp,其中5'UTR 66 bp,3'UTR216 bp,poly A尾长17 bp,并包含一个615 bp开放阅读框,编码205个氨基酸。GenBank登录号为KC588966。生物信息学分析表明,该蛋白是一种亲水性细胞质蛋白,包括10个磷酸化位点和8个功能位点;该蛋白属于NAC蛋白超级家族(NAC superfamily),包含NAC功能位点,同时包括一段UBA超家族结构域;其二级结构由α螺旋、无规则卷曲结构、延伸链和β转折组成;系统进化树分析表明苋菜amaNACA 2-like蛋白独立形成一个分支,但与其他物种间亲缘关系较近,说明NACA 2-like蛋白在生物进化过程中比较保守。研究amaNACA-2 like基因克隆和生物信息学分析,可为深入研究其功能奠定基础。The full-length amaNACA 2-like cDNA sequence was cloned using RT-PCR and RACE techniques and the bioinformatics was analyzed from in vitro plantlets of Amaranthus tricolor in this experiment. The full-length cDNA sequence amaNACA 2-like was 896 bp in length, containing an open reading frame of 615 bp （encoding 205 animo acids）, flanked by 66 bp 5＇ UTR and 216 bp 3＇ UTR that contained a 17 bp polyA. The accession number was KC588966 in GenBank. Bioinformatics analysis showed that the protein was hydrophilic cytoplasmic protein, with 10 phosphorylation sites and eight functional sites. The protein belongs to NAC superfamily with a NAC functional site. In addition, a UBA superfamily domain was contained in the sequence. The secondary structure was consisted of alpha helix, coil, strand chain and beta turn. The phylogenetic tree indicated that amaNACA 2-like protein constituted a branch alone, but had a close relationship with other species, which indicated NACA 2-like protein possesses high conservatism during species evolution. This study was helpful for further study of plant NACA 2-like functions.

关 键 词：苋菜 试管苗 amaNACA-2 LIKE 克隆 生物信息学 

分 类 号：S572[农业科学—烟草工业]