生物絮凝高活性菌株筛选及发酵优化  

Screening bioflocculant strains and optimization of fermentation

作　　者：彭桂香[1] 卢秋雁 孔慧清 李雄 万涛[3] 卢钰升[3] 谭志远[3] 

机构地区：[1]广东省微生物信号与作物病害防控重点实验室/华南农业大学资源环境学院,广东广州510642 [2]广东天辰生物技术有限公司,广东广州510070 [3]华南农业大学农学院,广东广州510642

出　　处：《华南农业大学学报》2014年第2期66-72,共7页Journal of South China Agricultural University

基　　金：973计划项目(2010CB126502);国家自然科学基金(31370052);广东省科技计划项目(2010B090400434;2012B010300018);农业部南方植物营养与肥料重点实验室和广东省养分资源循环利用与耕地保育重点实验室开放基金资助项目(TFS2011-04);广东省烟草专卖局资助项目(粤烟科[2012]26号)

摘　　要：【目的】从种植野生稻的稻田土壤中分离筛选对环境无污染、具有高效生物絮凝活性的菌株.【方法】采用VM培养基从药用野生稻中分离135个菌株,以发酵液对高岭土悬液絮凝效果为指标,筛选到絮凝活性高的菌株YH39.【结果和结论】16S rDNA序列分析表明,该菌株与越南伯克霍尔德菌Burkholderia vietnamiensis有98.6%的相似性.YH39产絮凝剂最佳碳源为葡萄糖,最佳氮源为硝酸钾.发酵动力学研究表明,菌株YH39发酵培养16 h,菌体浓度和絮凝活性达到最高值.对絮凝剂成分分析表明,絮凝剂为多糖,具有很好的耐热性,在60℃下加热30min,絮凝活性不改变.应用研究结果表明,此絮凝剂对3种印染废水均有很好的絮凝效果,不仅可以高效地降低废水的CODCr,还有很好的脱色效果.[Objective] This study aimed at isolating and screening of efficiently biological flocculation strains from the paddy field of the planted wild rice .[Method]A high bioflocculant strain YH 39 was ob-tained from the screening of kaolin suspension flocculation effects of 135 strains isolated from Oryza offici-nalis on VM medium.[Result and conclusion]16S rDNA sequences analysis showed that the strain YH39 was closely related to the known species Burkholderia cepacia with 98.6%similarity.The best car-bon and nitrogen sources for producing flocculation were glucose and potassium nitrate , respectively .The fermentation kinetics analysis showed that strain YH 39 reached the highest values of biomass concentra-tion and flocculating activity in 16 h.Flocculant composition analysis indicated that the bioflocculant components were polysaccharide with good heat resistance , which was heated at 60℃for 30 min without losing flocculating activity .The application of bioflocculation of three kinds of dyeing wastewater show that the fermentation liquid has good flocculating effects on dyeing wastewater with effective decolorization and reduces COD Cr in dyeing wastewater .

关 键 词：微生物絮凝剂 絮凝活性 菌株筛选 发酵优化 

分 类 号：X703.1[环境科学与工程—环境工程] Q931.9[生物学—微生物学]