乳腺癌组织原代培养细胞中的干细胞检测及其临床意义研究  

Study on the clinical significance and expression of breast cancer stem cells in breast cancer tissues of primary culture

作　　者：崔军威[1] 于志强[1] 刘晓岭[1] 韦伟[1] 

机构地区：[1]北京大学深圳医院乳腺外科,广东深圳518036

出　　处：《中国妇幼保健》2014年第7期1109-1112,共4页Maternal and Child Health Care of China

基　　金：深圳市科技局项目〔201102010〕

摘　　要：目的：①探讨具有干细胞标记特性的ALDHCD44＋细胞在乳腺癌组织及癌旁组织中的表达情况。②探讨乳腺癌ALDHCI）44＋细胞与肿瘤免疫组化指标ER、PR、Her2及Ki67之间的关系。方法：①实验组选取24例乳腺癌患者的术中新鲜癌组织，另取17例癌旁2cm组织标本作为对照。机械一酶方法消化组织、收集细胞悬液，并行肿瘤细胞原代培养，再经流式细胞仪检测ALDHCD4g＋细胞的含量。②利用统计学相关分析及￡检验方法了解乳腺癌原代细胞中ALDHhiCD44‘细胞与临床免疫组化指标ER、PR、Her2、Ki67之间相关性。结果：①24例浸润性乳腺癌组织中有22例原代培养成功（培养成功率为91．67％），17例癌旁组织中有14例能够培养出原代细胞（培养成功率为82．35％）。②ALDHhiCIM4＋细胞在癌组织原代培养的细胞中含量为（4．56±2．92）％（0．56％-10．30％），在癌旁组织原代细胞中含量为（0．15±0．06）％（0．05％-0．23％），两值相比差异有统计学意义（P〈0．05）。@ALDHhiCD44‘细胞与ER（P=0．065）、PR（P=0．107）、Her2（P=0．050）表达量之间差异无统计学意义，无相关性，与Ki67（P=0．007）表达量之间差异存在统计学意义，相关系数r=0．593。结论：①利用肿瘤组织原代细胞培养、检测方法，进一步证实ALDHhiCD44＋乳腺癌干细胞的存在，其在癌灶的表达量高于癌旁组织。②结合患者临床病理结果分析，显示乳腺癌干细胞ALDHhiCD44＋与Ki67之间存在相关性，与ER、PR、Her2指标之间无相关性。Objective： To explore the expression of ALDHh breast cancer stem cells in breast cancer tissues and cancer ad jacent tissues, and explore the relationship between ALDHhi CIM4 ＋ cells and ER, PR, Her2, Ki67. Methods： The fresh breast cancer specimens of 24 cases during surgery were collected as experimental group and the cancer adjacent tissue specimens of 17 cases were collect- ed as control group ; the specimens were digested by mechanical - enzyme method, cell suspension was collected to perform primary culture, and then the content of ALDHhi CIM4 ＋ cells were detected by flow cytometry ; the relationship between ALDHhl CD44 ~ cells and ER, PR, Her2, Ki67 was analyzed with t test. Results： Among 24 cases of invasive breast cancer tissues, primary culture was successful in 22 cases, the successful rate was 91.67% ; among 17 cases of cancer adjacent tissues, primary culture was successful in 14 cases, the successful rate was 82. 35% ; the contents of ALDHhl CD44 ＋ cells in invasive breast cancer tissues and cancer adjacent tissues were （4. 56 ± 2. 92 ） % （0. 56% - 10. 30% ） and （0. 15± 0. 06 ） % （ 0. 05 % - 0. 23 % ）, respectively, there was statistically significant difference （ P 〈 0. 05 ） ; there was no correlation between ALDHhiCIM4 cells and ER （P =0. 065） , PR （P =0. 107） , and Her2 （P =0. 050） ; but there was a sig- nificant correlation between ALDHhi CD44 cells and Ki67 expression （ P = 0. 007, r = 0. 593 ） . Conclusion ： The exitance of ALDHhi CIM4 cells were further confirmed through primary culture and detection of breast cancer cells, and its expression level in breast cancer tis- sues was higher than that in cancer adjacent tissues; the study showed that there was a correlation between ALDHhiCD44 cells and Ki67, but there was no correlation between ALDHhiCD44 cells and ER, PR, Her2.

关 键 词：乳腺癌 肿瘤干细胞 原代培养 免疫组化 

分 类 号：R737.9[医药卫生—肿瘤]