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作 者:何淼[1] 徐鹏飞[1] 赵保成[1] 李海芯[1] 李强[1] 张彦妮[1]
机构地区:[1]东北林业大学园林学院,黑龙江哈尔滨150040
出 处:《草业科学》2014年第2期243-249,共7页Pratacultural Science
基 金:中央高校基本科研业务费专项资金项目(DL12CA11);中央高校基本科研业务费专项资金项目(DL12CA17);黑龙江省青年基金项目(QC2009C52)
摘 要:以PEG-6000为渗透介质模拟干旱胁迫,对中国芒(Miscanthus sinensis)幼苗的生理特性进行了研究。随着PEG胁迫时间的延长,丙二醛(MDA)、相对电导率逐渐升高,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)、抗坏血酸(ASA)、谷胱甘肽(GSH)呈先升高后降低的趋势,但不同浓度PEG胁迫条件下,各指标的变化幅度不同。低浓度PEG(≤10%)各指标都能基本维持与对照相似的水平;高浓度PEG(≥20%)下,各指标与对照均差异显著,这说明中国芒幼苗能忍受低于20%的PEG胁迫,具有一定的抗旱能力,但浓度超过20%的PEG会对中国芒幼苗造成严重干旱胁迫,甚至导致其死亡。The effects of different concentrations of polyethylene glycol (PEG) solution intimidated water stress on physiological property of Miscanthus sinensis were studied. The results showed that the content of malondialdohyde(MDA) and relative conductivity increased, superoxide dismutase(SOD), peroxidase(POD), catalase (CAT), ascorbate dependent peroxidase (APX), glutathione reductase(GR), ascorbate(ASA) and glutathione(GSH) increased firstly and then decreased with PEG stress extension. However, the variation of each index was different under different PEG stress conditions. Each of indices could maintain similar levels with control (CK) in low PEG concentration (10% or less). These indices were significantly different with CK in high PEG concentration (20% or higher). These results showed that M.sinensis seedlings can stand less than 20% PEG stress and had a certain degree of drought tolerance. More than 20% PEG could bring M.sinensis seedlings severe drought stress and even death.
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