不同寒热药性中药对酵母致热大鼠的体温调节作用研究  

Effect of different cold and hot properties of TCM on thermoregulation of yeast induced fever rats

作　　者：万红叶[1] 孔祥英[2] 杨悦[2] 李晓敏[2] 林娜[2] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国中医科学院中药研究所中药理论与本草文献研究中心,北京100700

出　　处：《国际中医中药杂志》2014年第3期228-232,共5页International Journal of Traditional Chinese Medicine

基　　金：中国中医科学院自主选题研究课题（项目编号：Z02096）;国家自然科学基金重点项目（项目编号：No.30730111）

摘　　要：目的观察比较四种典型寒、热药性中药对酵母致热大鼠体温的调节作用及相关因子的影响。方法108只SD大鼠按照随机数字表法随机分为正常组、模型组、大黄组、黄连组、吴茱萸组和高良姜组各18只，背部皮下注射酵母混悬液建立大鼠发热模型。各组大鼠分别于造模前1h、造模后3．5h灌胃给药，正常组、模型组给予蒸馏水灌胃；其余各组给予相应药物水煎液灌胃。分别于造模后4h、8h和12h取血和下丘脑，通过酶联免疫吸附法检测血浆和下丘脑匀浆液中前列腺素E2（PGE，）、环磷酸腺苷（cAMP）和精氨酸加压素（AVP）的含量。结果注射酵母后4h，模型组大鼠体温开始升高，8h达高峰，大黄、黄连可显著降低发热大鼠体温（P〈0．01或0．05）；注射酵母后8h，模型组大鼠下丘脑的PGE，和cAMP含量[分别为（31．55±9．88）pg／mg、（0．17±0．03）pmol／mg]增高，血浆中AVP水平（0．14±0．02）pmol／ml降低，与正常组比较，差异有统计学意义（P〈0．05）。大黄和黄连可降低酵母致热大鼠下丘脑PGE，[分别为（113．65±18．60）pg／mg、（127．72±15．75）pg／mg]、cAMP[分别为（0．69±0．08）pmol／mg、（0．74±0．10）pmol／mg]含量，增加发热大鼠血浆中AVP含量[分别为（1．08±0．12）pmol／ml、（0．91±0．01）pmol／m1]，与模型组比较，差异有统计学意义（P〈0．01或0．05）；吴茱萸和高良姜对发热大鼠的体温和相关因子均无明显影响。结论寒性中药大黄、黄连能降低酵母致热大鼠的体温，其作用机制可能与调节下丘脑中PGE，、cAMP、血浆中AVP因子的含量有关；热性中药吴茱萸、高良姜无相关作用。Objective To compare the effect of 4 different cold and hot properties of traditional Chinese medicine （TCM） on body temperature and related factors of yeast induced fever rats, and discuss the thermoregulatory mechanism of cold and hot properties of TCM. Methods 108 male SD rats were randomly divided into a normal group, a yeast-induced group, a R. palmatum treated group, a C. chinensis treated group, a Euodia ruticarpa treated group, and a Alpinia officinarum Hance treated group, with 18 rats in each group. Pyrexia model was induced by injecting yeast suspension subcutaneously on rat. At the 4h, 8h and 12h after injection of yeast, the rats were sacrificed, and the blood and hypothalamus were collected. The levels of prostaglandin E2 （PGEz）, cyclic adenosine 3＇,5＇-monophosphate （cAMP） and arginine vasopressin （AVP） in hypothalamus and plasma were detected by ELISA assay. Results At the 4h after injection of yeast, the temperature of rats in the model group began to rise, and it reached the peak at 8h, while RheumpalmatumL and Coptis chinensis could significantly reduce the body temperature of yeast-induced rat （P〈0.01 or P〈0.05 ） . At 8h, the levels of PGE2 and cAMP in hypothalamus increased significantly [respectively （31.5± 9.88 ） pg/mg and （0.17±0.03） pmol/mg] compared with the normal group, while the level of AVP （0.14±0.02） pmol/ml in plasma reduced （P〈0.05）. Compared with model group, at 8h RheumpalmatumL and Coptis chinensis could significantly lowered PGE2 [respectively （113.6±18.60） pg/mg and （127.72 ±15.75） pg/mg, P〈0.01 or P〈 0.05], and cAMP [respectively （0.69±0.08） pmol/mg and （0.74±0.10） pmol/mg, P〈0.05] in hypothalamus, and increased AVP levels [respectively （1.08±0.12） pmol/ml and （0.91 ±0.01） pmol/ml, P〈0.05 or P〈0.01] in plasma. Euodia ruticarpa and Alpinia officinarum had no significant effect on both body temperature and the levels of inflammatory factors. Conclusion The two cold property traditional Chi

关 键 词：寒热药性 酵母 解热作用 前列腺素E2 环磷酸腺苷 精氨酸加压素 

分 类 号：R285.5[医药卫生—中药学] R285[医药卫生—中医学]