一种田头菇属真菌新记录种的生物学与产酶特性  

Biological characteristics and enzyme production of a newly recorded species of Agrocybe

作　　者：秦改娟 马济丰[1] 陈青君[2] 管博文 吴凡[1] 卫莹[1] 张国庆[1] 

机构地区：[1]农业部都市农业(北方)重点实验室,北京农学院生物科学与工程学院,北京102206 [2]北京农学院植物科学技术学院,北京102206

出　　处：《应用与环境生物学报》2014年第1期148-151,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(31200070);北京市本科生科学研究计划项目资助~~

摘　　要：田头菇是重要的经济食用菌类型,目前我国报道种仅有10个.为驯化栽培和开发利用新的田头菇属食用菌种质资源,以采集自北京延庆的我国新记录种菌核田头菇(Agrocybe tuberosa)GSM-12菌株为材料,研究了菌丝生长的最适碳源、氮源、C/N比、最适温度、最适pH值等生物学特性,以及菌丝体液体发酵产漆酶、蛋白酶、植酸酶和核糖核酸酶的特性.结果显示,菌丝生长的最适碳源为蔗糖,最适氮源为黄豆粉,最适C/N比为20/1-30/1,最适温度为24℃,最适pH为7.0.产酶特性研究显示,GSM-12菌株具有漆酶、植酸酶和核糖核酸酶活性,菌丝体中酶活较高,分别为(77.28±10.44)U/mg、(61.01±2.71)U/mg和(21.13±0.60)U/mg.本研究表明菌株GSM-12可以利用常规营养元素实现营养生长,同时菌丝体液体发酵具有良好的产酶特性.The genus Agrocybe is a very important economical mushroom with only 10 reported species in China. Agrocybe tuberose strain GSM-12 in the present study is a newly recorded species in China and was isolated from Yanqing District, Beijing. The goal of this work was to conduct further artificial domestication cultivation of the new mushroom species to explore its applications. Biological characteristics of mycelial growth and enzyme production in liquid fermentation were intensively studied. Results showed that the most favorable carbon and nitrogen sources were sucrose and soybean meal with optimal rate of 20/1-30/1 C/N, optimal temperature 24 ℃, and optimal pH 7.0. The liquid fermentation test showed that A. tuberosa, especially mycelia, produced enzymes including laccase （77.28 ± 10.44） U/mg, phytase （61.01 ± 2.71） U/mg and ribonuclease （21.13± 0.60） U/mg. It suggested that the strain can undergo good vegetative growth using normal nutrients, and that fermentation broth has considerably high enzyme activities.

关 键 词：菌核田头菇 生物学特性 产酶特性 漆酶 植酸酶 核糖核酸酶 

分 类 号：S646[农业科学—蔬菜学]