缺血性脑卒中大鼠大脑皮层EphrinB2信号表达变化的研究  

Expression of EphrinB2 Signal in Cerebral Cortex in Ischemic Stroke Rats

作　　者：郭心磊 孙芳玲[2] 艾厚喜[2] 张丽[2] 王文[2] 安宜[1] 

机构地区：[1]北京理工大学,北京市100081 [2]首都医科大学宣武医院药物研究室,北京市100053

出　　处：《中国康复理论与实践》2014年第2期129-132,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金(No.81173575;No.81373994);北京市自然科学基金(No.7062031);北京市教委科研基地-科技创新平台(No.111219);2011年北京市卫生系统高层次卫生技术人才(No.2011-3-097)

摘　　要：目的观察大鼠缺血性脑卒中后皮层中磷酸化EphrinB2的表达。方法将成年雄性Sprague-Dawley大鼠随机分为假手术组和模型组,每组12只。采用线栓法制作大鼠大脑中动脉阻塞(MCAO)缺血再灌注模型。利用Western blotting和免疫组化SP法检测两组大鼠脑皮层中磷酸化EphrinB2的表达。结果模型组大鼠大脑皮层的磷酸化EphrinB2表达量和阳性细胞数目均高于假手术组(P<0.05),并且血管内皮有磷酸化EphrinB2的表达。结论大鼠缺血性脑卒中后EphrinB2信号通路被激活。Objective To observe the expression of phosphorylated EphrinB2 in brain after focal cerebral ischemia/reperfusion in rats. Methods 24 male Sprague-Dawley rats were randomly divided into sham group （n=12） and model group （n=12）. The model group was mod-eled as middle cerebral artery occlusion and reperfusion with nylon monofilament suture, and then was assessed with Longa＇s score. The ex-pression of phosphorylated EphrinB2 in cerebral cortex was detected with immunohistochemistry and Western blotting. Results The expres-sion of phosphorylated EphrinB2 and the number of positive cells were significantly higher in the sham group than in the control group （P〈0.05）. It existed in the vascular endothelium in cerebral cortex. Conclusion EphrinB2 signaling pathway is activated in ischemic stroke.

关 键 词：脑卒中 大脑中动脉阻塞 缺血 再灌注 EPHRINB2 大鼠 EPHRINB2 

分 类 号：R743.3[医药卫生—神经病学与精神病学]