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作 者:王锦锋[1,2]
机构地区:[1]西北农林科技大学,陕西杨凌712100 [2]陇东学院农林科技学院,甘肃庆阳745000
出 处:《安徽农业科学》2014年第2期392-394,共3页Journal of Anhui Agricultural Sciences
基 金:甘肃省科技支撑计划项目:项目编号1011NKCM063
摘 要:[目的]测定核桃不同品种花粉的生活力,并研究其贮藏条件。[方法]采用固体培养基和液体培养基测定维纳、元丰、香玲、丰辉和当地核桃5个品种的花粉生活力,并对贮藏条件进行考察。[结果]固体培养基测定的花粉生活力普遍高于液体培养基0.98%~2.51%。5个品种均在2.5%蔗糖溶液中萌发率高;花粉最适宜的贮藏条件为-20℃、遮光。核桃不同品种的花粉生活力存在显著差异(P〈0.05);在同一培养基上,维纳花粉生活力最高为12.3%,其次是丰辉、元丰、香玲,当地核桃最低为3.9%。[结论]花粉生活力的测定以固体培养基较适合。同一培养基上,维纳花粉生活力最高。[ Objective ] To study different varieties of walnut pollen viability and storage conditions. [ Method ] Pollen vitality of 5 walnut culti- vars of Weina, Yuanfeng, Xiangling, Fenghui, Dangdihetao were determined by solid and liquid medium. [ Result] The results showed that the determination of solid medium of pollen vitality were higher than liquid medium at the level of 0.98% to 2.51%, so the pollen vitality of determi- nation in solid medium was more suitable ; five varieties high germination rate in 2.5% sucrose solution ; pollen optimum storage conditions for - 20 ℃, the shading; there exited significant deviation of pollen vitality of walnut cuhivars, the varieties in the same medium, Weina had the high- est vitality of pollen reached 12.3%, the second was Fenghui, Yuanfeng, Xiangling, minimum of local walnut was 3.9%. [ Conclusion] Solid medium is more suitable for determination of pollen viability. On the same medium, Weina pollen viability is the highest.
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