转化生长因子β1作用下绒毛膜癌JEG-3细胞c-myc mRNA表达  

Effect of transforming growth factorβ1 on c- myc mRNA expression in JEG- 3 choriocarcinoma cell

作　　者：毛晓丹[1] 谭宇思[1] 李玉红[2] 许倩[3] 刘绍晨[2] 张孔雁 

机构地区：[1]承德医学院研究生处,河北承德067000 [2]承德医学院教务处,河北承德067000 [3]承德医学院基础医学研究所,河北承德067000

出　　处：《临床和实验医学杂志》2014年第3期168-171,共4页Journal of Clinical and Experimental Medicine

基　　金：河北省自然科学基金资助项目(H2012406010)

摘　　要：目的观察在转化生长因子β1(TGF-β1)、-β1受体Ⅰ抑制剂(LY364947)和p38MAPK抑制剂(SB203580)作用下,绒毛膜癌JEG-3细胞中c-myc mRNA的表达变化。方法用5 ng/ml的TGF-β1以及1μM、3μM TGF受体Ⅰ抑制剂(LY364947)和1μM、3μM p38MAPK抑制剂(SB203580)作用JEG-3细胞,用qRT-PCR技术检测各组细胞中c-myc mRNA的表达差异。结果与正常对照组比较,5 ng/ml TGF-β1组细胞中c-myc mRNA的表达水平升高(P<0.05);p38 MAPK抑制剂(SB203580)和TGF-β受体Ⅰ抑制剂(LY364947)均抑制了c-myc mRNA的表达,且抑制作用与应用浓度呈正相关(P<0.05)。结论 c-myc作为TGFβ1/Smads通路的下游靶基因,其调控有赖于TGFβ1与受体Ⅰ的结合,同时,在绒毛膜癌JEG-3细胞中,TGF-β1介导的Smad途径与p38 MAPK信号转导存在交互作用。Objective To observe the impact of TGF - β1 ,TGF - β receptor1 inhibitor and p38 MAPK inhibitors on mRNA expression changes of c - myc in choriocarcinoma in JEG - 3 cell line. Methods The JEG - 3 choriocarcinoma cells were treated with 5 ng/ml TGF - β1, and TGF- β receptor 1 inhibitor （LY364947）, p38 MAPK inhibitors （SB 203580） at the concentration of 1 ~M, 3 ixM respectively, mRNA lev- els of c - myc was detected by qRT - PCR. Results The mRNA level of c - myc in 5 ng/ml TGF - β1 group increases, compared with control group （ P 〈0.05 ）. Blockade of TGF - β pathway and p38MAPK pathway both can attenuated and inhibited the expressions of c - myc in a dose - dependent manner（ P 〈 0.05 ）. Conclusion C - myc is a downstream target gene of TGF β 1/Smads pathway, and its expression level is reg- ulated by the combination of TGFβ1 and TGF - β receptor 1. Meanwhile, there~ an interaction between TGF - β1/smad signal pathway and p38 MAPK pathway in choriocarcinoma JEG - 3 cell line.

关 键 词：绒毛膜癌 JEG-3细胞 TGF-Β1 TGF-β1受体Ⅰ抑制剂 P38MAPK抑制剂 C-MYC 

分 类 号：R737.33[医药卫生—肿瘤]