免疫PCR方法快速检测饮料中的赤藓红  

Rapid detection of erythrosine in drinks by immuno-PCR

作　　者：龚强[1] 丁利[1] 肖家勇[1] 罗斯斯[2] 焦艳娜[1] 朱绍华[1] 文江为 付善良[1] 王利兵[1] 

机构地区：[1]湖南出入境检验检疫局技术中心食品安全科学技术湖南省重点实验室,湖南长沙410004 [2]上海出入境检验检疫局,上海200135

出　　处：《食品与机械》2014年第1期75-77,81,共4页Food and Machinery

基　　金：国家"十二五"科技支撑计划(编号:2011BAK10B05;2012BAD29B05);国家质检总局公益性专项(编号:201210036)

摘　　要：免疫PCR方法是结合免疫学和PCR原理的一种新型检测技术，具有高灵敏度、高自动化、操作简便等特点。研究采用PCR方法建立饮料中赤藓红快速检测方法。通过优化包被抗原浓度、探针DNA的量、抗体浓度等条件，结果显示：赤藓红的检测低限为10．0fg／g，线性浓度范围为0．05～50pg／g，果汁饮料中赤藓红的添加回收率为71．5％～112．6％，该方法简单、灵敏，适用于饮料中赤藓红的测定。Immuno-polymerase chain reaction（Immuno-PCR） is a novel technique with the characteristic of high sensitivity,high automa tion and easy operation. A rapid detection method of erythrosine was developed. After the optimization of the coating antigen concentra- tion, the amounts of probe DNA, and the antibody concentration, erythrosine detection limit was low as 10.0 fg/g, and the linear con centration range was 0.05 - 50 pg/g. The recoveries of fruit drinks ranged from 71.5% to 112.6%. This method was simple, sensitive, and suitable for the detection of erythrosine in drinks.

关 键 词：免疫PCR 赤藓红 饮料 

分 类 号：R96[医药卫生—药理学]