Cu^(2+)对生物膜及其胞外聚合物的影响  

Effect of Cu^(2+) on biofilm and extracellular polymeric substance

作　　者：胡学伟[1] 李姝[1] 荣烨[2] 江孟[1] 张燕[1] 李媛[1] 

机构地区：[1]昆明理工大学环境科学与工程学院,云南昆明650500 [2]昆明理工大学现代农业工程学院,云南昆明650500

出　　处：《化工学报》2014年第3期1062-1067,共6页CIESC Journal

基　　金：国家自然科学基金项目(50908109;51178208;51368024);云南省教育厅重点项目(2013Z123);昆明理工大学分析测试基金(20140549);云铜校企业合作项目(2013YT02)~~

摘　　要：利用自行设计的生物膜培养装置进行挂膜,通过向生物膜反应器中投加不同浓度的Cu2+,探讨生物膜上的胞外聚合物(extracellular polymeric substance,EPS)与生物膜去除金属铜之间的关系。研究结果表明:生物膜法较活性污泥法对Cu2+具有更好的耐受性;当Cu2+<2 mg?L?1,Cu2+会抑制生物膜分泌EPS,在2 mg?L?1<Cu2+<5mg?L?1时,生物膜分泌EPS的量增加,当Cu2+>5 mg?L?1,生物膜系统表现为严重的不稳定性;比较蛋白质(proteins,PN)/多糖(polysaccharide,PS)值发现,Cu2+对生物膜的抑制主要是对生物膜上胞外多糖的抑制,而生物膜对Cu2+的抵抗表现为分泌更多的胞外蛋白;EPS与Cu2+的富集率关系呈线性正相关。Through adding different concentration of Cue＋ in the biofilm reactor, the relationship between extracellular polymeric substance（EPS） secreted by biofilm and efficiency of copper removal by biofilm was studied. Biofilm tolerance of Cu^2＋ was better than activated sludge, but a high concentration of Cu^2＋ rapidly poisoned the biofilm. When Cu^2＋〈2 mg.L^-1, Cu^2＋ inhibited biofilm secretion of EPS. Concentration of 2 mg＇L-1 〈Cu^2＋〈5 mg＇L-a, Cu^2＋ would increase the amount of EPS, while Cu^2＋〉5 mg.L-1, the biofilm system displayed serious instability. Comparing proteins/polysaccharide （PN/PS） value showed that the biofilm suffered inhibition by Cu^2＋. The biofilm would inhibit more extracellular polysaccharide secretion, and the biofilm resistance of Cu^2＋ indicated that the biofilm would secrete more extracellular protein. The amount of EPS had a good correlation with the enrichment ratio of Cu^2＋.

关 键 词：生物膜 铜胁迫 活性 胞外蛋白 胞外多糖 

分 类 号：X703.1[环境科学与工程—环境工程]