三乙胺降解菌SYA-1的分离、降解性能与动力学  

Isolation and degradation characteristics of a triethylamine degrading strain SYA-1 and its kinetics

作　　者：沈敏雅[1] 胡江[1] 蔡舒[1] 蔡天明[1] 

机构地区：[1]南京农业大学资源与环境科学学院,南京210095

出　　处：《环境工程学报》2014年第3期1243-1249,共7页Chinese Journal of Environmental Engineering

基　　金：江苏省省级环保科技项目(201005);江苏省太湖治理科研课题(TH2012203)

摘　　要：从农药废水处理池的活性污泥中分离筛选得到1株高效三乙胺降解菌株SYA-1,根据菌株SYA-1的形态特征、生理生化特性和16S rRNA基因序列同源性分析,此菌株鉴定为Achromobacter sp.。菌株SYA-1能以三乙胺为惟一碳、氮源生长,并在24 h内完全降解200 mg/L的三乙胺。环境因素影响实验表明,在温度30℃,初始pH 7.0,NaCl浓度≤10 g/L条件下,菌株SYA-1生长良好且对三乙胺的降解效率最佳;金属离子对菌株生长和三乙胺降解的抑制程度表现为:Cu2+>Co2+>Ag+>Cd2+>Fe3+>Pb2+。菌株SYA-1降解三乙胺的动力学过程可用Haldane模型模拟,其参数为μmax=0.123h-1;K s=82 mg/L;K i=215 mg/L。为含三乙胺废水的生物降解提供了理论依据和菌株资源。A novel highly effective triethylamine（ TEA）-degrading bacterial strain,designated SYA-1,was isolated from an activated sludge used to treat pesticide wastewater. The strain was identified as Achromobacter sp. using an analysis of its 16S ribosomal RNA gene sequence（ 16S rRNA） and an examination of its morphological,physiological and biochemical properties. The strain consumed TEA as its sole carbon and nitrogen source,degrading 200 mg/L TEA to a non-detectable level within 24 h. By investigating environmental factors,the optimal conditions for the growth of strain SYA-1 and the TEA degradation were 30℃,initial pH of 7. 0,NaCl of 10g/L. Besides,heavy metal ions could inhibit the growth of strain SYA-1 and the degradation of TEA in different extents,the inhibitory effect in a descending order was： Cu2 ＋ Co2 ＋ Ag＋ Cd2 ＋ Fe3 ＋ Pb2 ＋. The kinetics process in the degradation of TEA by strain SYA-1 followed the Haldane model,and the parameters were： μmax=0. 123 h- 1; K s= 82 mg/L; K i= 215 mg/L. The results provide theoretical references and strain resources for the biodegradation of TEA in wastewater treatment plants.

关 键 词：三乙胺 无色杆菌属 分离鉴定 降解特性 动力学 

分 类 号：X172[环境科学与工程—环境科学]