苦荞异槲皮苷对人胃癌细胞SGC-7901增殖及凋亡的影响  

Effect of Isoquercetin from Fagopyrum tataricum on the Proliferation and Apoptosis of Human Gastric Carcinoma Cell Line SGC-7901

作　　者：李玉英[1] 赵淑娟[1] 白崇智[2] 张立伟[1] 王转花[1] 

机构地区：[1]山西大学化学生物学与分子工程教育部重点实验室,山西太原030006 [2]山西省中医药研究院中心实验室,山西太原030012

出　　处：《食品科学》2014年第3期193-197,共5页Food Science

基　　金：国家自然科学基金项目(31171659);山西省自然科学基金项目(2011011035-4);山西省高校科技开发项目(2012004)

摘　　要：从苦荞中提取制备异槲皮苷,研究其对人胃癌细胞SGC-7901增殖、凋亡、迁移和细胞周期的影响。将异槲皮苷作用于人胃癌SGC-7901细胞和人肾上皮细胞系293T,通过噻唑蓝法检测异槲皮苷对其增殖的影响;4’,6-二脒基-2-苯基吲哚(4’,6-diamino-2-phenyl indole,DAPI)荧光染色法观察细胞核的形态学变化;划痕擦伤迁移实验检测异槲皮苷对SGC-7901细胞迁移能力的影响;流式细胞术检测异槲皮苷对SGC-7901细胞凋亡及细胞周期的影响。结果表明:苦荞异槲皮苷可以抑制SGC-7901细胞的增殖,并呈时间和剂量依赖性,当用100μmol/L异槲皮苷作用细胞48 h后,对SGC-7901细胞的增殖抑制率达到35.92%,而对人肾上皮细胞系293T的增殖抑制率仅为3.15%;DAPI荧光染色法观察异槲皮苷处理细胞后,染色体凝聚,有凋亡小体产生;划痕擦伤实验显示,异槲皮苷能抑制SGC-7901细胞的迁移;流式细胞术检测结果表明,异槲皮苷可使G1和S期细胞减少,G2/M期细胞增多,且细胞凋亡率明显增加。综上所述,苦荞麦异槲皮苷能够诱导SGC-7901细胞发生凋亡,阻断细胞周期并抑制细胞增殖和迁移。Isoquercetin was extracted from Fagopyrum tataricum and investigated for its effect on cell proliferation, apoptosis and migration of human gastric carcinoma cell line SGC-7901. Cell proliferation was examined by using MTT assay. The morphological changes of SGC-7901 were observed by DAPI nuclear staining. The effect of Fagopyrum tataricum isoquercetin on cell migration was investigated by scratch-recovering tests in cell culture plates, as well as its effect on cell apoptosis by flow cytometry. As a result, MTT assay showed that the isoquercetin could inhibit the viability of SGC-7901 cells in time- and dose-dependent manners. SGC-7901 cell growth was inhibited by 35.92% or more at isoquercetin concentration of 100 μmol/L for 48 h, compared to only 3.15% for 293T cells under the same conditions of concentration and culture duration. DAPI nuclear staining showed chromosomal condensation and the production of apoptotic bodies. Scratch-recovering tests indicated that the isoquercetin could suppress cell migration. Flow cytometry showed that the number of cells at the G 1 phase and S phase decreased and increased at the G 2 /M phase and the apoptotic cell population exhibited a signif icant increase. Taken together, isoquercetin from F. tataricum can induce apoptosis, arrest cell cycle and inhibit cell proliferation and migration of SGC-7901 cells.

关 键 词：异槲皮苷 人胃癌细胞SGC-7901 细胞周期 凋亡 细胞迁移 

分 类 号：R965[医药卫生—药理学]