葡萄籽原花青素对顺铂导致H9c2细胞毒性的保护作用  

Protective Effect of GSPE on Cisplatin-Induced Nephrotoxicity in H9c2 Cells

作　　者：郭培培[1] 郭卓雨[1] 赵艳萌 高丽萍[1] 

机构地区：[1]北京联合大学应用文理学院生物活性物质与功能食品北京市重点实验室,北京100083

出　　处：《食品科学》2014年第3期213-216,共4页Food Science

基　　金：北京联合大学人才强校计划人才资助项目(20111101)

摘　　要：目的:研究葡萄籽原花青素对顺铂诱导大鼠H9c2心肌细胞损伤的影响并探讨其可能机理。方法:体外培养H9c2细胞,噻唑蓝法测定葡萄籽原花青素、顺铂对H9c2细胞生长的影响及葡萄籽原花青素对顺铂所致H9c2细胞毒性的影响,观察细胞的形态变化,并通过检测H9c2细胞及培养液中超氧化物歧化酶活性、丙二醛含量研究其可能机理。结果:葡萄籽原花青素能明显改善顺铂所致H9c2细胞形态变化,并抑制顺铂所致H9c2细胞及培养液中超氧化物歧化酶活性的降低、丙二醛含量的升高。结论:体外培养条件下葡萄籽原花青素能改善顺铂诱导的H9c2细胞的损伤,其作用机制可能与调高H9c2细胞的抗氧化能力有关。Aim： To explore the effect of grape seed proanthocyanidin extract（GSPE） on cis-diamminedichloroplatinum（CDDP）-induced damage in H9c2 cells and its mechanism. Methods： H9c2 cells were cultured in vitro. The effects of CDDP individually and in combination with GSPE on the growth of H9c2 cells were determined by MTT method. Meanwhile, the morp hologic change of the cells was observed. SOD activity and MDA content in both cells and medium were determined to explore its mechanism. Results： GSPE could improve CDDP-induced morphologic change and suppress the decrease in SOD activity as well as the increase in MDA content in H9c2 cells and culture solutions. Conclusion： GSPE can ameliorate CDDP-induced damage in H9c2 cells in vitro. Its mechanism may be correlated with increased antioxidant capacity in H9c2 cells.

关 键 词：葡萄籽原花青素 顺铂 H9C2细胞 抗氧化 

分 类 号：R318.0[医药卫生—生物医学工程]