MicroRNA-181b对衰老内皮细胞血管新生功能的影响  

Regulatory Role of MicroRNA-181b in the Angiogenesis of Senescent Endothelial Cells

作　　者：范婧尧 米雪楠[1] 陈宇[1] 贺淑君[2] 王雷[2] 惠汝太 张伟丽[1] 

机构地区：[1]北京协和医学院中国医学科学院国家心血管病中心阜外心血管病医院心血管疾病国家重点实验室心血管病基因研究室,北京市100037 [2]北京市海淀区妇幼保健院,北京市100080

出　　处：《中国分子心脏病学杂志》2014年第1期826-829,共4页Molecular Cardiology of China

基　　金：国家自然科学基金重大研究计划项目(91339101)

摘　　要：目的研究microRNA-181b(miR-181b)对衰老内皮细胞增殖、迁移和管腔形成等血管新生功能的影响。方法原代培养人脐静脉内皮细胞(Human umbilical vein endothelial cells,HUVECs),通过传代培养计算细胞群体倍增水平(Population doublings,PDL),建立HUVECs衰老模型(PDL44)。在PDL44 HUVECs中过表达或抑制miR-181b,分别用MTS法、划痕实验及成管实验检测内皮细胞增殖、迁移和成管功能。结果过表达miR-181b可抑制PDL44内皮细胞的增殖功能18%(P<0.001),减少内皮细胞迁移率40%(P=0.032);反之,给予miR-181b抑制剂可显著改善PDL44内皮细胞的迁移和增殖。给予血管内皮生长因子(Vascular endothelial growth factor,VEGF)刺激,未观察到miR-181b对PDL44内皮细胞成管功能的调控作用。结论抑制miR-181b可提高衰老内皮细胞的增殖和迁移能力,提示它可作为一个新的靶点,调节内皮细胞的损伤修复和血管新生。Objective To study the role of miR-181b in senescent endothelial cells angiogenesis. Methods Primary human umbilical vein endothelial cells （HUVECs） were cultured and population-doubling levels （PDLs） were calculated during passages. PDL44 was identified as senescent HUVECs. MiR-181b mimics and inhibitor were separately transfected into PDL44 HUVECs, and then the abilities of cell proliferation, migration and tube formation were assayed by MTS test, scratching test, and tube formation test, respectively. Results In vitro analysis of PDL44 HUVECs, overexpression of miR- 181 b inhibited endothelial proliferation by 18% （P〈0.00I） and migration by 40% （P=0.032）. In contrast, miR- 18b inhibitor can significantly increased endothelial proliferation and migration. The regulatory role of miR-181 b in endothelial tube formation was not observed in response to VEGF stimulus. Conclusion Inhibition of miR-18 lb can enhance proliferation and migration of senescent endothelial cells, which it may serve as a target in regulating senescent endothelial repair and angiogenesis.

关 键 词：细胞衰老 血管新生 内皮细胞功能 MICRORNAS 

分 类 号：R363[医药卫生—病理学]