UPLC-ELSD法测定柴达木栽培和野生枸杞子中水溶性糖含量  

Determination of Water-Soluble Sugar in the Wild and Cultivated Fructus Lycii from Qaidam Basin Using UPLC-ELSD

作　　者：曹静亚[1,2] 迟晓峰[1] 谭亮[1] 胡风祖[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810008 [2]中国科学院研究生院,北京100049

出　　处：《天然产物研究与开发》2014年第2期233-237,共5页Natural Product Research and Development

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-EW-J-26)

摘　　要：建立超高效液相-蒸发光散射(UPLC-ELSD)法测定柴达木栽培和野生枸杞子中的鼠李糖、木糖、果糖、葡萄糖和蔗糖的方法。以ACQUITY UPLC BEH Amide柱为分析柱,乙腈和水(各含0.2%TEA)作为流动相,流速为0.15 mL/min,柱温为45℃,ELSD为检测器,漂移管温度为50℃,N2流量为40 psi。研究发现,枸杞子中的水溶性糖主要为果糖和葡萄糖,两种糖的检测限分别为0.86和0.64μg/mL;平均回收率分别为91.8%(RSD=1.66%)和89.6%(RSD=2.55%)。该方法操作简单、效率高、重现性好、结果准确可靠,适用于枸杞子中水溶性糖的含量测定。测定结果表明,柴达木栽培枸杞子具有很整齐的品质优良性,而野生枸杞子品质差异很大,适宜于选择性的开发。An uhra-pefformanee liquid chromatographycoupled with evaporative light scattering detector（UPLC-ELSD） method was developed to determine the contentsofrhamnose,xylose,fructose,glucose and sucrose in the wild and cultivat- ed Fructus Lycil from Qaidam Basin. The carbohydrates were separated on an ACQUITY UPLC BEH Amide column u- sing acetonitrile-water（0.2% TEA in both solvents） as mobile phases. The flow rate was 0. 15 mL/min with the column temperatureset at 45 ℃. The drift tube temperatureof the ELSDwas set at 50 ℃ and the flowrate of N2 was set at40 psi. It was found that fructose and glucose were the major water-soluble sugar in the wild and eultivatedFructusLycii from Qaidam Basin. The limit of detection of fructose and glucose were 0.86 μg/mL and 0.64μg/mL,respectively;the aver- age recoveries （ n = 9） were 91.8% （ RSD = 1.66 % ） for fructose and 89.6% （ RSD = 2.55% ） for glucose. The method- issensitive, reliable and accurate. It isapplicableto determinewater-soluble sugar in FructusLycii. The result shows that quality of the eultivatedFruetusLyciifrom Qaidam Basiuis neatly excellent. However, the quality of wildFructusLyciihas significant differences ,which is suitable for the selective exploitation.

关 键 词：超高效液相色谱 蒸发光散射检测 柴达木 枸杞子 水溶性糖 

分 类 号：O657.7[理学—分析化学]