人肝细胞癌4号染色体高频率杂合子丢失的研究  

Frequent loss of heterozygosity on chromosome 4 in human hepatocellular carcinoma

作　　者：李晓明[1] 丁敏[1] 赖宝山[2] 刘宗石[3] 

机构地区：[1]安徽省立医院病理科 [2]香港中文大学外科学系 [3]香港中文大学医学院病理解剖及细胞学系外科学系

出　　处：《中华医学杂志》2001年第1期37-40,共4页National Medical Journal of China

基　　金：香港政府大学拔款委员会研究基金资助! (2 14 0 12 2 )

摘　　要：目的　为克隆新的肝细胞癌 (HCC)相关肿瘤抑制基因提供位点依据。方法　使用 4号染色体的 12个微卫星DNA多态性标志 ,分析了 48例原发性HCC的杂合子丢失 (LOH)。结果　 44 %(2 1/4 8)和 6 3% (30 /4 8)的肿瘤组织中至少有 1个位点的LOH分别发生在 4p和 4q。丢失图排列鉴定了两个独立的共同丢失片段 (CDR)。第 1个定位在 4q2 2 q2 5的CDR位于D4S392和D4S16 2 5位点之间 ;第 2个定位在 4q2 7 q31的CDR位于D4S16 2 5和D4S16 5 2位点之间。结论　至少有两个肿瘤抑制位点存在于 4q。Objective Few previous studies have shown high frequency of loss of heterozygosity (LOH) on chromosome 4q in hepatocellular carcinoma (HCC). We define more clearly the deletion regions that may harbor the putative tumor suppressor genes in HCC. Methods Forty eight cases of HCC and their corresponding non tumor liver tissues were investigated with 12 microsatellite polymorphic markers for LOH. Results Twenty one of 48 (44%) and 30 of 48 (63%) tumors showed LOH on at least one locus on the short and the long arms respectively. Two distinct common deleted regions (CDR) with different patterns of deletion were identified. The first CDR was located on 4q22 q25, between loci D4S392 and D4S1625. In addition, 17 of 27 (63%) of the informative tumors showed LOH on this region with locus D4S406 and constituted the highest rate of LOH on chromosome 4. The second CDR was located at 4q27 q31, between loci D4S1625 and D4S1652. Conclusion There are at least two tumor suppressor loci on 4q.

关 键 词：肝肿瘤 染色体 杂合子 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]