HERG蛋白对胃癌细胞凋亡及细胞周期分布的影响  

Effects of HERG Protein on Cell Cycle and Apoptosis of Gastric Cancer Cells

作　　者：邵晓冬[1] 张永国[1] 陈江[1] 王金玲[1] 郭晓钟[1] 

机构地区：[1]中国人民解放军沈阳军区总医院消化内科,沈阳市110016

出　　处：《广西医学》2014年第2期146-150,共5页Guangxi Medical Journal

基　　金：国家自然科学基金资助项目(30400204)

摘　　要：目的研究人eag相关基因(HERG)蛋白表达对胃癌细胞凋亡及细胞周期分布的影响。方法构建HERG-siRNA载体;将构建的载体转染胃癌细胞SGC7901,氨基糖苷类抗生素G418进行转染细胞的稳定筛选并克隆化,从蛋白和电流水平鉴定转染细胞。采用流式细胞仪检测胃癌细胞凋亡情况;采用透射电子显微镜技术观察胃癌细胞超微结构的变化;采用流式细胞仪检测胃癌细胞细胞周期分布的变化。结果 HERG-siRNA可以阻断胃癌细胞中的HERG电流,可以诱导胃癌细胞出现凋亡;在透射电子显微镜下观察到典型的凋亡细胞的形态学改变;HERG-siRNA可以阻止胃癌细胞由G1期进入S期。结论 HERG-siRNA可抑制HERG蛋白及相应钾电流,从而促进胃癌细胞凋亡,抑制胃癌细胞G1/S期转化,具有潜在的抗癌作用。Objective To explore the effects of human ether-a-go-go-related gene （ HERG ） protein on cell cycle and apoptosis of gastric cancer cells .Methods HERG-siRNA vector was constructed and transfected into gastric cancer cell SGC7901 ,G418 was applied to screening and cloning the transfected cells which were verified with HERG current and protein .Flow cytometry was used to assess the apoptosis of gastric cancer cells;Transmission electron microscope was used to observe the ultrastructure of gastric cancer cells ;The cell cycle distribution of gastric cancer cells was investigated by flow cytometry .Results HERG-siRNA eliminated the HERG current in gastric cancer cells and induced apoptosis in gastric cancer cells;There were typical changes of apoptosis under transmission electron microscope;HERG-siRNA inhibited gastric cancer cells entering into S phage from G 1 phage.Conclusion HERG-siRNA can inhibit HERG protein and potassium current as well as the progression of G 1/S of gastric cancer cells ,and induce apoptosis of gastric cancer cells .HERG -siRNA has potential anti-cancer effects on gastric cancer cells .

关 键 词：胃癌 人eag相关基因蛋白 钾离子通道 细胞凋亡 细胞周期 钾电流 

分 类 号：R735.2[医药卫生—肿瘤]