环氧化酶2基因Gly587Arg多态与原发性肝癌发病风险的相关性  

The relationship between Gly587Arg variant of COX-2 gene and the primary liver cancer

作　　者：王光霞[1] 付占昭[2] 邵莎莎[1] 宋琴琴[3] 饶娟[4] 刘英文[4] 张志[3] 

机构地区：[1]承德医学院,067000 [2]秦皇岛市第一医院肿瘤放化疗科 [3]河北联合大学附属唐山市工人医院肿瘤放化疗科 [4]河北联合大学生命科学学院

出　　处：《中国综合临床》2014年第2期141-143,共3页Clinical Medicine of China

基　　金：国家自然科学基金资助（81101483）;河北省杰出青年科学基金资助项目（H2012401022）

摘　　要：目的 探讨中国汉族人群环氧化酶2 （COX-2）基因Gly587Arg多态与原发性肝癌发病风险的关系.方法 选取原发性肝癌患者270例及健康对照者540名,提取外周血淋巴细胞DNA,利用PCR-限制性片段长度多态性方法进行基因分型,以多变量Logistic回归分析比值比（OR）及其95％ CI.结果 在肝癌组及对照组只发现587Gly/Gly和Gly/Arg两种基因型,未发现587Arg/Arg基因型.肝癌组587Gly/Gly和Gly/Arg的基因型频率分别为91.5％ （247/270）、8.5％ （23/270）,对照组分别为96.5％（521/540）、3.5％ （19/540）,多变量Logistic回归分析显示,587Gly/Arg基因型携带者原发性肝癌发病风险是587Gly/Gly基因型携带者的2.56倍,其95％ CI为1.37 ～4.79（P =0.003）.结论 中国汉族人群中COX-2基因Gly587Arg多态与原发性肝癌的发病风险相关.Objective To explore the association of COX-2 Gly587Arg polymorphism with the risk of primary liver cancer.Methods Two hundred and seventy patients with primary liver cancer and 540 health people were selected as our subjects.DNA were extracted from peripheral blood lymphocytes,and genotypes were measured by polymerase chain reaction-restriction fragment length polymorphism method.Odds ratios（OR） and 95％ confidence intervals（CI） were estimated by logistic regression.Results Two kinds of genotype （587Gly/ Gly and Gly/Arg） were found in all participants.No one carried 587Arg/Arg genotype.Among primary liver cancer patients,91.5％ （247/270,） 8.5％ （23/270） of individuals carried 587Gly/Arg and Gly/Arg genotype,which was significantly higher than that of controls （96.5％ （521/540,） 3.5％ （19/540））.Multivariate Logistic regression analysis showed that individual carried 587Gly/Arg genotype had an increased risk of developing primary liver cancer （OR =2.56,95％ CI =1.37-4.79,P =0.003） compared with 587Gly/Gly carriers.Conclusion COX-2 Gly587Arg polymorphism is a risk factor for primary liver cancer in Han.

关 键 词：原发性肝癌 环氧化酶2 单核苷酸多态 基因分型 

分 类 号：R735.7[医药卫生—肿瘤]