低氧对人喉鳞癌Hep-2细胞凋亡的影响及其机制探讨  

Effect of hypoxia on apoptosis of human laryngeal cancer Hep-2 cell line and its mechanism

作　　者：董凯峰[1] 张翠红[2] 宋冬梅[1] 吕欣[1] 张继华[1] 薛海涛[1] 赵玉玲[1] 牛英豪[1] 

机构地区：[1]河北医科大学第一医院,石家庄050031 [2]白求恩国际和平医院

出　　处：《山东医药》2014年第8期12-13,16,共3页Shandong Medical Journal

基　　金：河北省医学科学研究重点课题计划项目(20120049)

摘　　要：目的观察低氧对人喉鳞癌Hep-2细胞凋亡的影响,并探讨其机制。方法将体外培养的人喉鳞癌细胞分为常氧组、低氧组。流式细胞仪检测细胞凋亡率,RT-PCR及Western blot法分别检测各组细胞低氧诱导因子α(HIF-1α)、赖氨酰氧化酶(LOX)mRNA及蛋白。结果常氧组细胞凋亡率13.86%±0.12%、低氧组5.13%±0.67%,P<0.05。与常氧组比较,低氧组细胞中HIF-1α、LOX蛋白及其mRNA表达增加(P均<0.05),且二者表达呈正相关(r均为0.862,P均<0.05)。结论低氧可抑制Hep-2细胞凋亡,该作用可能与其上调细胞中HIF-1α、LOX表达有关。Objective To investigate the effect of hypoxia on apoptosis of human laryngeal cancer Hep-2 cell line and its mechanism.Methods Human laryngeal squamous carcinoma cells cultured in vitro were divided into the normoxic,and hypoxic groups.The apoptosis was detected by flow cytometry,and the expression of hypoxia-inducible factor （HIF）-1 a,lysyl oxidase （LOX） protein and mRNA was detected by Western blot and RT-PCR,respectively.Results Hypoxia significantly increased the expression of HIF-1 a protein and mRNA in the Hep-2 cells and the LOX protein and mRNA expression was up-regulated.Flow cytometry showed that the apoptosis rates were （13.86 ± 0.12） ％ in the normoxic group and （5.13 ± 0.67）％ in the hypoxia group,statistically significant difference was found between the two groups （P ≤0.05）.Conclusion Hypoxia inhibits the apoptosis of Hep-2 cells,whose mechanism may be associated with the up-regulation of HIF-1a and LOX expression.

关 键 词：喉肿瘤 HEP-2细胞 低氧 凋亡 低氧诱导因子 赖氨酰氧化酶 

分 类 号：R73-3[医药卫生—肿瘤]