腺病毒介导miRNA-138抑制人胃癌BGC-823细胞增殖的机制研究  

Inhibitive effect of adenovirus-mediated miRNA-138 on proliferation of gastric carcinoma cell line BGC-823 in vitro

作　　者：范亚川[1] 梁光萍[2] 陈先华[2] 胡春燕[3] 邹利全[1] 李学成[1] 陈陵[1] 

机构地区：[1]中国人民解放军三二四医院消化内科,重庆400020 [2]第三军医大学西南医院烧伤研究所,重庆400038 [3]第三军医大学新桥医院肿瘤生物治疗中心,重庆400037

出　　处：《重庆医学》2014年第6期679-680,683,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(30900679);重庆市自然科学基金资助项目(cstc2011jjA10111;cstc2011jjA10114;cstc2012jjA10145)

摘　　要：目的探讨负载miRNA-138(miR-138)的重组复制缺陷型腺病毒对人胃癌BGC-823细胞株增殖能力的影响及可能的机制。方法将负载miR-138的重组腺病毒感染人胃癌BGC-823细胞株,采用荧光实时定量PCR检测miR-138表达丰度;采用CCK8方法检测细胞生长曲线;采用Transwell方法检测细胞侵袭能力。结果与Ad-LacZ对照组相比,感染Ad-miR138后,BGC-823细胞内miR-138表达丰度明显升高,差异有统计学意义(1.07±0.07 vs.4.89±0.45,P<0.05);第6天吸光度明显下降,差异有统计学意义(0.52±0.06 vs.0.77±0.06,P<0.05);细胞侵袭能力亦显著下降,差异有统计学意义(32.00±11.00 vs.56.00±12.00,P<0.05)。结论 miR-138体外可显著抑制人胃癌BGC-823细胞增殖及侵袭能力。Objective To investigate the effect of the replication-deficient adenovirus carrying miR-138（Ad-miR138） on the pro-liferation of human gastric cancer cell line BGC-823 and the possible mechanism .Methods The human gastric cancer cell line BGC-823 was infected with Ad-miR138 .Then the expression level of miR-138 was measured by RT-PCR .The growth curve of BGC-823 was measured using CCK-8 method .The ability of cell invasion was measured using Transwell chambers .Results After infected with Ad-miR138 ,the expression of miR-138 in BGC-823 cells was up -regulated significantly （1 .07 ± 0 .07 vs .4 .89 ± 0 .45 ,P〈0 .05） .Absorbance of the 6th day decreased significantly （0 .52 ± 0 .06 vs .0 .77 ± 0 .06 ,P〈0 .05） ,and the invasion ability was de-creased obviously （32 .00 ± 11 .00 vs .56 .00 ± 12 .00 ,P〈0 .05） .Conclusion miR-138 can effectively suppress the proliferation and invasion of human gastric cancer cell line BGC-823 in vitro .

关 键 词：腺病毒科 胃肿瘤 微RNAS 

分 类 号：R735.2[医药卫生—肿瘤]