荧光猝灭法测定抗坏血酸  

Determination of Ascorbic Acid by Fluorescence Quenching Method

作　　者：翟好英[1] 李琼[1] 

机构地区：[1]内江师范学院化学化工学院,四川省内江市东兴区东桐路705号641112

出　　处：《光谱实验室》2014年第2期226-229,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：在酸性介质中，当用280nm的光激发时，罗丹明类染料丁基罗丹明B和罗丹明6G分别在590和560nm处产生1个较强的荧光峰。加入抗坏血酸后，由于丁基罗丹明B和罗丹明6G分别在静电引力作用下与抗坏血酸形成离子缔合物，从而使体系的荧光猝灭。在优化实验条件下，在1．00-26．0μg／mL（丁基罗丹明B体系）或2．00-20．0μg／mL（罗丹明6G体系）范围内，体系的荧光强度△F与抗坏血酸浓度之间均呈良好的线性关系，其检出限为分别为0．916或0．0563μg／mL。该方法简单快速，且选择性好，可直接用于维生素C药片中抗坏血酸含量的测定。In acidic medium, as excited with 280nm light, rhodamine dyes such as butyl rhodamine B and rhodamine 6G have a strong fluorescence peak at 590 and 560 nm respectively. After added to ascorbic acid, butyl rhodamine B and rhodamine 6G react respectively with ascorbic acid to form ion-association complexes acted on the electrostatic force, thus the fluorescence intensity of the system quenches. Under the optimized experimental conditions, there is a good linear relationship between fluorescence intensity and the ascorbic acid concentration in the range of 1.00-26.0 or 2.00-20.0μg/mL with the limit of detection of 0.916 or 0.0563μg/mL. The method is simple, rapid and selective, and can be directly used for the determination of ascorbic acid content of tablet.

关 键 词：丁基罗丹明B 罗丹明6G 抗坏血酸 荧光猝灭 

分 类 号：O657.32[理学—分析化学]