高温厌氧消化中底物浓度对病原指示微生物杀灭的影响  

Effect of Substrate Concentration on Pathogen Indicators Inactivation During Thermophilic Anaerobic Digestion

作　　者：操宏庆[1] 章菲菲[1] 李健[1] 童子林[1] 胡真虎[1] 

机构地区：[1]合肥工业大学土木与水利工程学院,合肥230009

出　　处：《环境科学》2014年第3期1016-1020,共5页Environmental Science

基　　金：国家自然科学基金项目(51078122);教育部新世纪优秀人才支持计划项目(NCET-11-0624);国家水体污染控制治理科技重大专项(2012ZX07103-001)

摘　　要：剩余污泥中含有大量病原微生物，土地利用之前必须进行适当的处理，降低病原微生物的数量，以降低环境风险．通过研究在不同的底物浓度条件下，剩余污泥高温厌氧消化（55℃）过程对病原指示微生物的杀灭效果，分析厌氧消化前后污泥中病原指示微生物数量变化的原因．结果表明，随着底物浓度的增加，VFAs浓度和累积产甲烷量增加．在底物浓度为28-84g·L^-1、温度为55℃、持续28d的高温厌氧消化过程中，上清液中总大肠杆菌下降2．0-3．0个数量级、粪大肠杆菌下降1．8-3．3个数量级；消化后污泥中总大肠杆菌和粪大肠杆菌杀灭率均达到99％以上；且上清液中和消化污泥中均未检测到沙门氏菌的存在．在研究底物浓度范围内，当底物浓度大于45g·L^-1时，消化液和污泥中总大肠杆菌和粪大肠杆菌的杀灭效果下降．Because excess sludge contains high density of pathogens, it has to be treated to reduce pathogens before being disposed for land application. In this study, the effect of substrate concentration on pathogen inactivation during thermophilie anaerobic digestion was investigated. The results show that, with the increase of substrate concentration, VFAs and cumulative methane production increased. The density of total coliforms in the suspension liquid has a 2. 0-3.0 orders of magnitude decline and fecal coliforms has 1.8-3.3 orders of magnitude decline after 28 days thermophilic anaerobic digestion at substrate concentration of 28-84 g.L^-l and temperature of 55°C. More than 99% of total eoliforms and fecal eoliforms have been killed after 28 days digestion. Salmonella spp. was not detected in the suspension and solid after anaerobic digestion. When substrate concentration was higher than 45 g.L^-1 , the inactivation of total coliforms and fecal coliforms declined.

关 键 词：厌氧消化 剩余污泥 总大肠杆菌 粪大肠杆菌 沙门氏菌 

分 类 号：X703[环境科学与工程—环境工程]