对硝基苯胺耐盐降解菌S8的筛选及特性研究  

Isolation and Characterization of a Halotolerant p-nitroaniline Degrading Strain S8

作　　者：宋彩霞[1] 邓新平[1] 厉阗[1] 肖伟[1] 

机构地区：[1]西南大学植物保护学院,重庆400716

出　　处：《环境科学》2014年第3期1176-1182,共7页Environmental Science

摘　　要：从江苏农药厂活性污泥中筛选出1株能以对硝基苯胺为唯一碳源和氮源生长代谢的耐盐降解菌S8.通过对其形态特征、生理生化特性以及16S rDNA序列分析,确定为枯草芽孢杆菌(Bacillus subtilis).研究表明,该菌株具有较强的对硝基苯胺降解能力.当温度31℃和pH 6.0时,S8在72 h内对60 mg·L-1和120 mg·L-1对硝基苯胺的降解率分别为65.6%和55.8%.在高盐条件下,S8仍有较强的降解能力,当盐含量为7%时,对硝基苯胺降解率为49.5%(72 h);当盐含量为10%时,对硝基苯胺降解率为27.4%(72 h).利用LC-MS法分析降解产物时,共发现6种分子量不同的化合物,其中两种为苯酚及对苯二酚.此为首次关于对硝基苯胺耐盐菌株的研究报道,菌株S8可用于含有对硝基苯胺的高盐工业废水的微生物修复.A bacterial strain S8 was isolated from pesticide-contaminated sludge, which showed ability of utilizing p-nitroaniline as the sole source of carbon and nitrogen for growth. Based on the morphology, the physiological and biochemical characteristics, and the 16S rDNA sequence analysis, the strain was identified as Bacillus subtilis. Strain S8 showed high efficiency in p-nitroaniline degradation. 65.6% and 55.8% ofp-nitroaniline （60 mg.L-1 and 120 mg.L-1 ） were degraded by this strain within 72 hours under the optimal conditions of 31°C and pH 6.0. Besides, strain $8 degraded 49.5% p-nitroaniline in 7% NaC1 and 27.4% p-nitroaniline in 10% NaC1 （72 h） , which showed that the strain $8 had a high salinity tolerance. When the LC-MS method was used for identification of the biodegradation products, six kinds of degradation products were found, two of which were identified as phenol and hydroquinone. To date, this is the first report on the degradation of p-nitroaniline by Bacillus subtilis. These results suggest that $8 could be a potential candidate for treating p-nitroaniline-contaminated saline wastewater.

关 键 词：枯草芽孢杆菌 对硝基苯胺 高盐 降解特性 降解产物 

分 类 号：X172[环境科学与工程—环境科学]