TIP30基因表达对吉非替尼体内抑瘤作用的影响研究  

Correlation of TIP30 gene expression and the antitumor effect of gefitinib in the treatment of lung cancer in vivo

作　　者：陈锦章[1] 黄维[2] 郑大勇[1] 阮健[1] 陈逢生[1] 李爱民[1] 

机构地区：[1]南方医科大学南方医院肿瘤内科,广东广州510515 [2]顺德第一人民医院肿瘤科,广东顺德528300

出　　处：《海南医学》2014年第4期472-474,共3页Hainan Medical Journal

基　　金：广东省自然科学基金(编号:S2012010009392);南方医院院长基金(编号:2011C001)

摘　　要：目的探讨TIP30基因的表达与吉非替尼体内抑瘤作用的相关性。方法选取小鼠24只,随机分为实验组和对照组,每组12只。两组小鼠均经皮下种植肺癌细胞获得肿瘤模型,实验组通过沉默TIP30基因建立TIP30低表达模型,对照组裸鼠则不作任何处理,利用实时定量PCR和Western blot方法检测两组TIP30基因的表达情况。对两组小鼠未用吉非替尼10 mg/d进行处理,15 d后观察两组小鼠的体内肿瘤抑制程度。结果成功建立小鼠肺癌模型,实验组TIP30表达情况显著低于对照组,两组表达情况比较差异具有统计学意义(P<0.01)。对两组小鼠利用吉非替尼进行处理后,在第5天两组的瘤体平均重量未见显著差异,但第7天及第15天实验组瘤体重量显著低于对照组(P<0.05),病理切片显示实验组肿瘤抑制效果明显强于对照组。结论 TIP30基因对吉非替尼的体内抑瘤作用具有促进作用。Objective To investigate the correlation ofTlP30 gene expression and the antitumor effect ofge- fitinib in the treatment of lung cancer in vivo. Methods Twelve mice were randomly divided into experimental group and control group. Tumor model were established in two groups by subcutaneous injection of lung cancer cells. TIP30 low-expression model was established by silencing TIP30 genes in the experiment group; however, there was no any treatment in the control group. Then we used the real-time quantitative PCR and western blot method to detect the TIP30 gene expression. Gefitinib were used in both groups in a concentrate of 10 mg/d. As a result, both the anatomy and pathological effect of tumor inhibition were found after 15 days. Results The lung cancer models in mice was successful established. Real-time quantitative PCR and western blot confirmed that expression of TIP30 in experimen- tal group was significantly lower than that in control group （P〈O.O1）. After the first five days there was no statistical difference between groups in the average weight of tumor on the use of gefitinib, while there were significant statisti- cal differences between groups at the first 7 days and 15 days （P〈0.05）. Pathological results also showed that the tu- mor inhibitory effect in experimental group was significantly stronger than that in control group. Conclusion TIP30 gene can have a facilitating role in the antitumor effect in vivo.

关 键 词：TIP30 肺癌 表皮生长因子受体 体内研究 

分 类 号：R-332[医药卫生]