腔内弹力蛋白酶压力灌注联合腔外氯化钙浸润构建腹主动脉瘤大鼠模型  

A novel rat model of abdominal aortic aneurysm set up by combination of intraluminal elastase-infusion and extraluminal calcium chloride exposure

作　　者：曹旭[1] 王嫕 秦彦文[1] 

机构地区：[1]首都医科大学附属北京安贞医院北京市心肺血管疾病研究所心血管生物研究室,100029

出　　处：《中国医药》2014年第2期225-229,共5页China Medicine

基　　金：国家自然科学基金（81100222,81270389）

摘　　要：目的 联合应用弹力蛋白酶和氯化钙浸润的方法建立病理学特征符合腹主动脉瘤特点的大鼠肾下型腹主动脉瘤模型.方法 选取雄性Sprague-Dawle大鼠的左肾动脉远心端1 cm腹主动脉段给予腔内弹力蛋白酶（40 U/ml）和腔外氯化钙（0.5 mol/L）处理（模型组,n=9）,灌注组（n=6）（弹力蛋白酶和氯化钙溶液用0.9％氯化钠注射液代替）和假手术组（n=6）（仅正常开腹游离血管）作为对照组.分别于术前、术后3、7和10 d超声检测腹主动脉（瘤）直径变化.术后10 d取材固定,游标卡尺测量腹主动脉（瘤）直径,行组织切片苏木精-伊红染色和弹力蛋白Elastin yon Gieson（EVG）染色,计算机图像半定量分析比较3组血管壁厚度,弹力纤维含量参数的变化.结果 每组手术均在20 min内完成,围手术过程无技术失误和死亡.术后10 d,模型组腹主动脉直径以及扩张率分别为（2.75 ±0.49）mm、（68.4±22.6）％,高于灌注组[（1.86±0.16）mm,（6.3±3.7）％]和假手术组[（1.81±0.11）mm,（4.6±3.4）％].模型组、灌注组和假手术组术前、术后3、7和10 d动脉内径依时间变化分别为（1.68 ±0.18）mm,（2.37±0.26） mm,（2.69±0.34）mm,（2.81±0.38）mm;（1.71 ±0.15）mm,（1.79±0.21）mm,（1.83±0.19）mm,（1.82±0.24） mm;（1.66 0.19）mm,（1.64 0.14）mm,（1.63 ±0.22） mm,（1.61 ±0.13）mm.其中模型组动脉直径依时间变化明显,灌注组和假手术组2组无明显变化.苏木精-伊红染色示术后10 d模型组血管壁厚度为（136 ±6） μm明显小于灌注组的（177±5）μm和假手术组的（178±6）μm,灌注和假手术组2组差异无统计学意义（P＜0.05）.弹力蛋白EVG染色示模型组弹力蛋白含量为（5.8±2.9）％明显低于灌注组的（17.1±6.4）％和假手术组的（16.7±7.1）％,上述结果灌注组和假手术组2组差异无统计学意义.结论 联合应用腔内弹力蛋白酶灌注和腔外氯化钙浸润处理可�Objective To introduce a new,simple technique using a combination of intraluminal elastaseinfusion and extraluminal calcium chloride exposure to set up abdominal aortic aneurysm （AAAs） that simulates human aneurysms.Methods Experimental models of AAAs were created in rats by a 20-minute application of intraluminal elastase （30U） and extraluminal calcium chloride （0.5 mol/L） in the 1 cm segment of infrarenal abdominal aorta （model group,n =9）.A single application of elastase （Saline infusion group,n =6） or calcium chloride （sham group,n =6） was used as control.The aorta in each group was measured by ultrasonic detection before and after operation （3 d,7 d and 10 d） and was harvested at 10 d.Vascular wall thickness,lastolytic activity and histology in the treated aorta were evaluated among the three groups.Results The surgical procedure in each group was similarly completed for approximate 20 minutes and performed without any technical failure or operative death.At the 10th day,the artery diameter and the dilation ratio were （2.75 ± 0.49） mm and （68.4 ±22.6） % in model group,（1.86 ± 0.16） mm and （6.3 ± 3.7） % in saline infusion group,（1.81 ± 0.11） mm,（4.6± 3.4）% in sham group,respectively.The wall thickness in model group（136 ± 6）μm was significantly higher than that in saline infusion group（177 ±5）pm and in sham group（178 ±6）μm.Less elastin content in the aortic wall was observed in model group.According to the time course of artery size measured by ultrasonic detection before and after operation （3,7 and 10 d）,the artery diameter of the model group changed more significantly than other 2 groups.Conclusions The rat AAA model using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure is simple and easy to perform.It is highly reliable and reproducible to create a saccular aneurvsm similar to human AAAs.

关 键 词：实验性腹主动脉瘤 弹力蛋白酶 氯化钙 大鼠 

分 类 号：R732.21[医药卫生—肿瘤]