产油酵母菌高产培养基的营养成分优化  

Optimization of Nutritional Components of High Yield Medium of Oleaginous Yeast Strain

作　　者：马勇[1] 图雅[1] 刘晓光[1] 

机构地区：[1]包头师范学院生物科学与技术学院,内蒙古包头014030

出　　处：《湖北农业科学》2013年第23期5832-5835,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31160254);校内青年科学研究基金项目(BSYKJ2011-17)

摘　　要：以产油酵母菌Y1为供试菌株,通过单因素及正交试验对其产脂发酵培养基进行优化。结果表明,最优培养基组成为葡萄糖85 g/L、蛋白胨40 g/L、(NH4)2SO410.00 g/L、MgSO4·7H2O 0.300 g/L、Na2HPO43.0 g/L、KH2PO42.5 g/L。按照营养成分最优组合,100 mL三角瓶装液量30 mL,液体种子接种量10%,摇床转速160 r/min,温度28℃,得到的产油酵母菌Y1生物量为20.25 g/L,生物量比优化前提高了46.5%。Using the oleaginous yeast Y1 as the experiment strain,single factor experiments and orthogonal experiment of liquid fermentation medium were optimized.The optimal medium were glucose 85 g/L,peptone 40g/L,（NH4）2SO4 10.00 g/L,MgSO4·7H2O 0.300 g/L,Na2HPO4 3.0 g/L,KH2PO4 2.5 g/L.According to the optimum combination of nutritional components,liquid volume in 100 mL flask was 30 mL,seed inoculation of liquid was 10％,shaking speed was 160 r/min,temperature was 28 ℃,the cell biomass of oleaginous yeast Y1 was 20.25 g/L,the cell biomass increased by 46.5％ after the optimization.

关 键 词：产油酵母 营养成分 产脂 发酵 优化 

分 类 号：S963.327[农业科学—水产养殖]