PMA-LAMP法对克氏螯虾样品中沙门氏菌的快速检测  

Application of PMA-LAMP in Rapidly Detecting Salmonella in Cambarus Clarkii

作　　者：张毅[1] 胡定金[1] 付云洁 魏辉 

机构地区：[1]湖北省农业科学院农业质量标准与检测技术研究所,武汉430064 [2]湖北泰扬生物科技有限公司,武汉430070

出　　处：《湖北农业科学》2013年第23期5854-5857,共4页Hubei Agricultural Sciences

基　　金：湖北省农业科技创新中心项目(2011-620-000-001-3);湖北省农科院青年科学基金项目(2012NKYJJ19)

摘　　要：采用环介导等温扩增技术(PMA-LAMP)对克氏螯虾(Cambarus Clarkii)样品中沙门氏菌进行快速检测,旨在建立一种快速简便、灵敏度高、特异性强的检验方法。分别采用PMA-LAMP、PCR检测方法对沙门氏菌进行检测。结果表明,PMA-LAMP法检测限为2×10 CFU/mL,PCR法和GB/T 4789.4-2008检测限为2×103CFU/mL。采集湖北省省内60份市售的克氏螯虾样品,分别采用PMA-LAMP检测方法、PCR检测方法及国家标准方法(GB/T 4789.4-2008)对样品进行检测,PMA-LAMP方法检出6例沙门氏菌,阳性率为10%;PCR检测方法检出4例沙门氏菌,阳性率为6.7%;国家标准方法检出4例沙门氏菌,阳性率为6.7%。Loop-mediated isothermal amplification for rapid detection of Salmonella in samples of crayfish （Cambarus clarkii）was develeped to provide a quick and easy,highly sensitive and specific testing methods.PMA Loop-mediated isothermal amplification detection method,PCR detection method and national standard（GB/T 4789.4-2008） were used to isolate and identification of Salmonella.The results showed that the detection limit of the PMA-LAMP assay was 2×10 CFU/mL,indicating the sensitivity of PCR was 2×103 CFU/mL.PMA-LAMP method,PCR method and national standard （GB/T 4789.4-2008） were used to detect crayfish samples.PMA-LAMP method detected six cases with the positive rate of 10％.PCR method detected four cases with the positive rate of 6.7％.National standard method detected four case with the positive rate of 6.7％.

关 键 词：PMA-LAMP法 沙门氏菌(Salmonella) 克氏螯虾(Cambarus Clarkii) 

分 类 号：TS207.4[轻工技术与工程—食品科学]