邻苯二甲酸二丁酯的收缩血管作用及其机制  

Vasoconstriction effect of di-n-butyl phthalate and its underlying mechanism

作　　者：蒋昀[1,2] 夏强[1] 

机构地区：[1]浙江大学基础医学院生理系 [2]中国人民解放军42集团军75752部队

出　　处：《中国药理学通报》2014年第2期199-202,共4页Chinese Pharmacological Bulletin

基　　金：浙江省自然科学基金资助项目(No J20110334)

摘　　要：目的研究邻苯二甲酸二丁酯（di—n—butylphthalate，DBP）对大鼠动脉血管的作用，并探讨其可能机制。方法采用离体大鼠胸主动脉环灌流模型，观察DBP对动脉血管的收缩效应及其内皮的参与l生，并观察相关信号途径工具药的影响。结果DBP（10^-5-10-mol·L^-1）浓度依赖性收缩血管环，且去内皮血管比内皮完整血管收缩更明显（P〈0．05）；无Ca2＋“液中，DBP（10^-3mol·L^-1）引起的去内皮血管收缩明显低于正常K-H液中的血管收缩（P〈0．01），与对照组比较无差异；维拉帕米（10^-7mol·L^-1）预处理后，DBP（10^-3mol·L^-1）引起的血管环收缩幅度低于单用DBP组（P〈0．01）；一氧化氮合酶抑制剂左旋硝基精氨酸甲酯（L-NAME，10^-6mol·L^-1）预处理内皮完整主动脉环，引起的血管收缩幅度明显高于单用DBP组（P〈0．01），而前列环素合成酶抑制剂吲哚美辛（Indo，10^-5mol·L^-1）预处理对血管收缩幅度无明显影响。结论DBP具有引起血管收缩作用，初步机制可能涉及细胞膜电压门控性钙通道及钙离子内流，NO可能参与DBP的血管反应过程。Aim To investigate the effect of di-n-butyl phthalate （DBP） on rat arteries and its underlying mechanism. Methods The tension of the isolated Sprague-Dawley rat thoracic aorta rings perfused with different concentrations of DBP was measured to detect the contraction effect of the drug on rat arteries, where the participation of endothelial cells and relative signal transduction pathway was also checked. Results The DBP （ 10-5 . 10-3 tool . L-1 ） contracted the thoracic aorta rings with their endothelium preserved or removed in a concentration-dependent manner. The vasocon- striction of endothelium-free （ - E） aorta rings was more obvious than that with intact endothelium （ ＋ E ） （P 〈 0. 05）. In calcium-free solution, the contraction amplitude of - E aorta ring induced by DBP （ 10 -3 tool . L-1） was apparently smaller than that in normal perfusion solution （ P 〈 0. 01 ） , but showed no signifi-cant differences in the control group. Pretreated withverapamil, the aorta rings showed smaller contraction amplitude induced by DBP （ 10-3 tool . L-1） than that of the DBP group （P 〈 0.01 ）. Pretreated with nitric oxide synthase inhibitor （L-NAME, 10-4 tool . L-1 ） , the contraction amplitude was increased in intact endo- thelium（ ＋ E） aorta rings induced by DBP （10-3 tool ~ L-~ ） （P 〈0. 01 ）, when pretreated with indometha-cin （ 10-5 mol . L-1 ）, it had no effect when compared with that of the DBP group. Conclusions DBP has vasoconstricting effect on rat arteries. The underlying mechanism of the DBP action may be related to the sar- colemma voltage-gated calcium channels and calcium influx. Nitric oxide may participate in the reaction of DBP.

关 键 词：邻苯二甲酸二丁酯 胸主动脉 血管收缩 钙通道 内皮 一氧化氮 

分 类 号：R-332[医药卫生] R322.12