肿瘤相关巨噬细胞在结肠肿瘤中的浸润及意义  

Infiltration of tumor-associated macrophages and its significance in colonic neoplasm

作　　者：白明华[1] 司君利[2] 亓玉琴[1] 纪莉莎[1] 崔京远[3] 

机构地区：[1]青岛大学医学院附属青岛市市立医院消化内一科,山东青岛266011 [2]青岛大学医学院附属青岛市市立医院急诊科,山东青岛266011 [3]青岛大学医学院附属青岛市市立医院普外科,山东青岛266011

出　　处：《中国现代普通外科进展》2014年第1期24-27,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:了解结肠肿瘤组织中肿瘤相关巨噬细胞(TAMs)的浸润情况,并探讨其浸润程度的临床意义。方法:采用免疫组织化学染色的方法,用CD163标记巨噬细胞,光学显微镜下计数阳性染色细胞。结果:结肠癌组织中TAMs计数高于结肠腺瘤组织,结肠腺瘤组高于癌旁正常组织(P均<0.05),TAMs浸润程度与肿瘤的分化程度、浸润深度、淋巴结和远处转移等有关(P均<0.05)。结论:TAMs在结肠癌组织中浸润较多,且在其发生发展中起重要作用,并促进其侵袭、转移,可作为临床评估结肠癌病情的新指标。Objective： To find out the infiltration of tumor associated macrophages （TAMs） in colonic neoplasm tissue, and discuss its clinical significance. Methods： lmmunohistochemical SP method was used to detect the expression of CD163 protein in 53 cases of colonic cancer,20 cases of colonic adenoma,15 cases of normal colonic tissue（5 cm form the cancer）, and count the quantity of positive staining ceils. Results： The infiltration of TAMs in colonic cancer was higher than that in the colon adenoma and normal group（P〈0.05）, and it was associated with the degree of differentiation, depth of invasion and lymph node metastasis（all P〈0.05）. Conclusion： The tumor associated macrophages were much infiltrated in colonic cancer, and it played important roles in the development and promotion. It could promote the invasion and the metastasis of colonic cancer, and be a new evaluation for colonic neoplasm.

关 键 词：结肠肿瘤 肿瘤相关巨噬细胞 免疫组织化学 

分 类 号：R735.35[医药卫生—肿瘤]