不同途径摄取糖皮质激素大鼠骨髓基质干细胞生物活性研究  

Biological activity in bone marrow stromal cells from rats through different methods for administration of glucocorticoid

作　　者：魏秋实[1] 邓伟民[1] 何伟[2] 王海彬[3] 唐红宇[3] 江晓兵[2] 李子祺[3] 

机构地区：[1]广州军区广州总医院康复科,广东省广州市510010 [2]广州中医药大学第一附属医院骨科 [3]广州中医药大学中医骨伤科重点实验室

出　　处：《中国骨与关节损伤杂志》2014年第2期160-163,共4页Chinese Journal of Bone and Joint Injury

基　　金：国家自然科学基金(81302994;81273778;kbc110114k28);广东省科技计划省国际合作项目(2012B050600026);广东省自然科学基金(S2013040014927)

摘　　要：目的探讨不同途径摄取糖皮质激素对大鼠骨髓基质干细胞(BMSCs)生物活性的影响。方法 48只5个月龄SD大鼠随机分成正常组、口服激素组、肌注激素组,每组16只。分别于应用激素后9和12周,每组随机抽取8只麻醉后取左股骨检测骨密度(BMD);第12周结束后取右股骨,无菌下取骨髓培养BMSCs,MTT检测细胞增殖,AKP活性检测成骨能力,油红O染色观察成脂能力。结果激素干预9周后,口服组和肌注组股骨BMD低于正常组(P<0.05),口服组和肌注组差异无统计学意义(P<0.05);干预12周后,口服组和肌注组股骨BMD继续下降,肌注组BMD下降更明显。口服组与肌注组大鼠BMSCs早期增殖能力及AKP活性比正常组高,口服组最高;分化中后期增殖能力和AKP活性比正常组低,脂滴比正常组多,肌注组更明显。结论长期口服与肌注激素均能引起骨量丢失,肌注组更明显。应用激素后大鼠BMSCs增殖与成骨能力比正常大鼠弱,肌注激素大鼠BMSCs成脂能力比口服组强,说明不同途径摄取激素造成大鼠不同程度骨量丢失可能与其对BMSCs分化方向的影响程度有关。Objective To explore the effect of different methods of administration of the glueocorticoid on the biological activity in rat bone marrow stromal cell. Methods Forty eight 5-month SD rats were randomly divided into 3 groups： normal group, oral steroid group（OR group） and intramuscular steroid group（IM group）, 16 rats per group. After treatment for 9 and 12 weeks, eight rats were selected in each group, and under general anesthesia, bone mineral density （BMD） was measured in the left femur. At the end of 12 weeks, the right femur was collected to extract bone marrow for BMSCs culturing under sterile conditions, MTT Assay for proliferation, ALP activity assay for osteogenesis ability, Oil Red O staining for adipogenesis ability were performed. Results After treatment for 9 weeks, compared with normal group, the BMD of OR and IM group were obviously decreased（P 〈0.05）. There was no statistical significance between OR and IM group（P 〈0.05）. After treatment for 12 weeks, the BMD of OR and IM group were continuously decreased, IM group was more obvious. Compared with normal group, the ability of proliferation and ALP activity in OR and IM group were obviously increased, and OR group was the highest. At the late stage in differentiation, the ability of proliferation and ALP activity in OR and IM group were obviously decreased, and the ability of adipogenesis were obviously increased, IM group was more obvious. Conclusion Long-term oral and intramuscular steroid can induce bone mass loss, and bone mass loss in IM group is more obvious. The ability of proliferation and osteogenesis in rat with applying steroid BMSCs are weaker than normal rat BMSCs, the ability of adipogenesis in IM group is stronger than OR group BMSCs. The inordinate loss of the bone mass occurre in different ways to ingest the steroid rat partially due to its effects degree on the differentiation of bone marrow stromal cells.

关 键 词：糖皮质激素 骨髓基质干细胞 生物活性 骨密度 

分 类 号：R-33[医药卫生]