APC^(min/+);Mac-1^(-/-)小鼠模型的构建  

Establishment of APC^(min/+);Mac-1^(-/-) mice model

作　　者：雷岩[1] 章倩倩[1] 胡曦文[1] 刘红英[1] 郑凌云 顾取良[2] 何晓东[1] 王丽京[1] 

机构地区：[1]广东药学院血管生物学研究所,广州510006 [2]广东药学院基础学院

出　　处：《中国医疗前沿》2013年第23期29-30,共2页China Healthcare Innovation

基　　金：国家自然科学基金(编号:31271455;31200896;31100852;31200861);广东省医学科研基金(编号:B2012181);广东省自然科学基金(编号:S2012040007658)

摘　　要：目的构建APCmin/+;Mac-1-/-小鼠模型。方法将自发肠腺瘤(APCmin/+)模型同β2家族整合素Mac-1缺失的小鼠(Mac-1-/-)进行杂交,剪小鼠尾巴抽提DNA,然后用PCR法鉴定小鼠的基因型。结果将APCmin/+小鼠与Mac-1-/-小鼠杂交3代后,成功构建了APCmin/+;Mac-1-/-小鼠模型。结论将APCmin/+小鼠与Mac-1-/-小鼠杂交3代后成功建立了APCmin/+;Mac-1-/-小鼠模型,进一步扩大种群。的表达,并且这种作用能被L-精氨酸所抑制。Objective To establish the APC^min/＋;Mac-1^-/- mice. Methods Female PSGL-1 K0 mice were mated with male APC^min/＋ mice and male progeny carrying the APEmin/＋ transgene. Mice were genotyped by PCR. Results After female Mac-1 K0 mice were mated with male APC^min/＋ mice for three generations, APC^min/＋;Mac-1^-/- mice were established. Conclusion APC^min/＋;Mac-1^-/- mice were obtained and propagated through APC^min/＋ mice being mated with MAC-1 KO mice.

关 键 词：结直肠肿瘤 β2 整合素 MAC-1 动物模型 

分 类 号：R735.3[医药卫生—肿瘤]