TREK-1激动剂亚麻酸对星形胶质细胞谷氨酸诱导电流和摄取谷氨酸的影响  

Effect of TREK-1 agonist lineonic acid on astoglial glutamate transporter current and glutamate uptake capacity

作　　者：刘阳[1] 孙谦[1] 杨锦珊[1] 王伟[1] 谢敏杰[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《卒中与神经疾病》2014年第1期3-6,共4页Stroke and Nervous Diseases

基　　金：国家自然科学基金(No.30971007);国家自然科学基金重点项目(No.81030021)

摘　　要：目的观察双孔钾通道TREK-1激动剂亚麻酸对星形胶质细胞谷氨酸诱导电流和摄取谷氨酸的影响。方法应用大鼠海马脑片膜片钳技术研究TREK-1激动剂亚麻酸对星形胶质细胞谷氨酸诱导电流的影响;原代培养大鼠皮层星形胶质细胞,采用免疫荧光双标法检测TREK-1在星形胶质细胞的表达,采用谷氨酸浓度检测法观察TREK-1激动剂亚麻酸对星形胶质细胞摄取谷氨酸的影响。结果 TREK-1在星形胶质细胞上表达丰富,谷氨酸可诱导星形胶质细胞产生内向电流,与对照组相比,给予TREK-1激动剂亚麻酸干预后星形胶质细胞谷氨酸诱导电流显著增加(P<0.05),并显著增强原代培养的星形胶质细胞摄取谷氨酸能力(P<0.05)。结论 TREK-1在星形胶质细胞上表达,TREK-1激动剂亚麻酸显著增加星形胶质细胞谷氨酸诱导电流,增强原代培养的星形胶质细胞摄取谷氨酸能力,提示TREK-1活性改变与星形胶质细胞平衡缓冲功能密切相关。Objective To investigate the effect of TREK-1 channel agonist lineonic acid on astroglial glutamate transporter current and glutamate uptake capacity. Methods The expression of TREK-1 in cultured astrocytes was analyzed by double immunofluorescence staining. The effect of TREK-1 channel agonist lineonic acid on astoglial glutamate transporter current in hippocampal slice was studied using whole-cell-patch-clamp recording. The concentration of glutamate in primary cultured astrocytes was detected by colorimetric assay. Results TREK-1 was expressed on astrocytes abundantly. Compared with the control groups, astroglial glu tamate transporter current in hippocampal slice was significantly enhanced after treatment with lineonic acid. Meanwhile, glutamate uptake capacity was significantly increased after treatment with lineonic acid. Conclu-sions TREK-1 immunoreactivity was expressed on astrocytes. TREK-1 channel agonist lineonic acid can re markably enhance the astroglial glutamate transporter current in hippocampal slice and glutamate uptake capac ity in primary cultured astrocytes. Our results indicate that TREK-1 activity is closely related with astrocytic homeostatic function.

关 键 词：TREK-1 亚麻酸 谷氨酸诱导电流 谷氨酸摄取 

分 类 号：R743[医药卫生—神经病学与精神病学]