经典型霍奇金淋巴瘤中大细胞与小细胞亚系生物学特性比较  

Comparison of biological characteristics of big cell sublines and small cell sublines in classical Hodgkin's lymphoma cell lines and its significance

作　　者：李锋[1] 黄作平[1] 黄学平[1] 王志强[1] 朱梅刚[1] 赵彤[1] 

机构地区：[1]南方医科大学基础医学院病理学系,广东广州510515

出　　处：《中华肿瘤防治杂志》2014年第3期174-177,182,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(81071941)

摘　　要：目的:探究经典型霍奇金淋巴瘤细胞系L428中大小细胞亚系生物学特性差异及其意义。方法:应用有限稀释法分离出L428中大小细胞亚系并进行传代培养,分别命名为L428-big和L428-small细胞。免疫细胞化学实验检测L428中大小细胞亚系CD15和CD30表达差异,MTT实验检测L428中大小细胞亚系增殖能力的差异;流式细胞术检测L428中大小细胞亚系的凋亡差异。结果:成功分离出L428细胞株中大小细胞亚系并进行传代培养;免疫组化显示L428-big细胞的CD15及CD30表达明显强于L428-small细胞;MTT实验显示,L428-big细胞和L428-small细胞之间增殖能力差异有统计学意义,F=565.273,P<0.001,L428-small细胞增殖能力高于L428-big细胞,P<0.001;流式细胞术显示,L428-big细胞的凋亡率为(3.3±0.1)%,明显多于L428-small细胞(0.4±0.1)%,P<0.001。结论:L428-small细胞增殖能力强,凋亡少,为经典型霍奇金淋巴瘤细胞起源的进一步研究提供一定的实验基础。OBJECTIVE：To investigate differences of biological characteristics of big and small cell sublines in classi- cal Hodgkins lymphoma cell lines L428 and its significance. METHODS： Limited diluted method was used to separate the big and small cell sublines apart in cell lines of L428 which were named as L428 big and L428-small cell lines. Immunohis tochemisty was applied to detect the expression of CD15 and CD30 in cell lines of L428-big and L428-small. MTT assay was used to determine the differences of proliferation ability in cell lines of L428-big and L428-smalL Apoptosis was ana- lyzed by flow cytometry in cell lines of L428-big and L428-small. RESULTS： L428 cell lines were successfully separated with cell lines of L428-big and L428-small;Immunohistoehemistry showed the expression of CD15 and CD30 in L428-big cells were significantly stronger than that in L428-small cells;MTT assay showed that the differences between L428-big cells and L428-small cells were statistically significant （F= 565. 273, P〈0. 001）, with much higher multiplication capacity in L428-samll cells than that of L428-big cells （P〈0. 001） ;Flow cytometry showed more apoptotic cells in L428-big cells （3.3±0. 1）% than that of L428-small cells（0.4±0. 1）% （P〈0.00l）. CONCLUSION： The L428-small cell lines show higher multiplication capacity and less apoptotic cells, which is expected to provide the experimental basis for further study of the origins of classical Hodgkin lymphoma ceils.

关 键 词：L428细胞株 大小细胞亚系 有限稀释法 霍奇金淋巴瘤 

分 类 号：R733.1[医药卫生—肿瘤]