Nicotiflorin对人乳腺癌MCF-7细胞增殖及凋亡的影响  

Effect of Nicotiflorin in Inhibiting Proliferation and Inducing Apoptosis of Human Breast Cancer Cells MCF-7

作　　者：范春梅[1] 许婉卿[2] 史海鸿[3] 施清泉[4] 

机构地区：[1]福建医科大学附属第二医院中心实验室,泉州362000 [2]福建医科大学附属第二医院口腔科,泉州362000 [3]福建医科大学附属第二医院肿瘤外科,泉州362000 [4]福建医科大学附属第二医院普外科,泉州362000

出　　处：《福建医科大学学报》2013年第6期345-349,共5页Journal of Fujian Medical University

基　　金：泉州市科技项目(2010Z34;2012B7)

摘　　要：目的探讨Nicotiflorin(Kaempferol 3-O-β-rutinoside,山奈酚-3-O-芸香糖苷)对人乳腺癌MCF-7细胞的增殖抑制,以及诱导细胞凋亡和对细胞周期的影响。方法采用MTS法检测细胞存活率,计算IC50值;流式细胞仪检测细胞周期及细胞凋亡;Western-blot检测DNA损伤标记蛋白γ-H2AX及凋亡相关蛋白Bcl-2的变化。结果 Nicotiflorin能剂量依赖性地抑制人乳腺癌MCF-7细胞的增殖,IC50值为28.2μmol/L。流式细胞仪检测结果显示,Nicotiflorin使MCF-7细胞周期阻滞于G2/M期,不同浓度的Nicotiflorin作用48h后细胞凋亡率均增加。Western-blot检测发现,Nicotiflorin处理后,磷酸化的H2AX(γ-H2AX)随时间依赖性地增加,提示Nicotiflorin诱导DNA双链断裂从而使细胞阻滞于G2/M期,同时Nicotiflorin可能通过抑制抗凋亡蛋白Bcl-2的表达诱导细胞凋亡。结论 Nicotiflorin能抑制人乳腺癌MCF-7细胞的增殖并诱导其凋亡。Objective To study the effect of Nicotiflorin （Kaempferol 3-O-β-rutinoside） on prolif-eration ,cell cycle distribution and apoptosis of human breast cancer cells MCF-7 . Methods M TS assay was performed to detect the viability of MCF-7 cells and flow cytometry were used to observe the effect of Nicotiflorin on cell cycle distribution and apoptosis . Western blot was applied to deteted γ-H2AX at the DNA double-strand breaks （DSBs） of chromatin as well as Bcl-2 expression . Result Nicotiflorin inhibi-ted the proliferation of MCF-7 cells in a dose-dependent manner and IC50 is 28 .2 μmol/L . Nicotiflorin arrested MCF-7 cells at G2/M phase and induced apoptosis . Western blot showed that Nicotiflorin in-duced DSBs and dow n-regulated the expression of Bcl-2 in a time-dependent manner . Conclusion Nicoti-florin has the biological activity to inhibit proliferation ,cell cycle of human breast cancer MCF-7 cell as well as to induce cell apoptosis .

关 键 词：山柰酚 黄酮类 乳腺肿瘤 细胞凋亡 肿瘤细胞  培养的 细胞周期 

分 类 号：R737.9[医药卫生—肿瘤]