p16、p15蛋白在小儿急性淋巴细胞白血病的表达及临床相关性研究  

Expression of p16 and p15 Proteins in Acute Lymphoblastic Leukemia of Children

作　　者：胡宛如[1] 张瑞英[1] 郭承吉[1] 王立娥[2] 都姝妍[2] 

机构地区：[1]中国医科大学第一临床学院儿科,沈阳110001 [2]中国医科大学第一临床学院中心实验室

出　　处：《中国医科大学学报》2001年第1期64-65,68,共3页Journal of China Medical University

摘　　要：目的 :探讨 p16、p15蛋白在急性淋巴细胞白血病 (AL L)发病中的意义。方法 :对 2 3例 AL L 细胞进行间接免疫荧光染色 ,用流式细胞仪检测细胞的荧光强度 ,间接反映 p16、p15蛋白水平。结果 :2 3例 AL L 患儿 p16蛋白阴性 10例 ,p15蛋白阴性 8例 ,p16、p15蛋白均阴性 6例。 3例 T- AL L 中 p16、p15蛋白皆阴性 2例 ,13例Non T- AL L中 ,p16蛋白阴性 6例 ,p15蛋白阴性 5例。高白细胞组的 p16、p15蛋白表达阳性率低于非高白细胞组 ,差异有显著意义 (P <0 .0 5 ) ,HR- AL L 组 p16、p15蛋白阳性表达低于 SR- AL L 组 ,差异有显著意义 (P <0 .0 5 )。结论 :p16、p15蛋白参与了部分急性淋巴细胞白血病的发病 ,p16。Objective:Our aim was to To clarify the roles of p16 and p15 proteins in the genesis of acute lymphoblastic leukemia(ALL).Methods:Twenty three samples of ALL were studied by the method of indirect immunofluorescence.Flow cytometer was used to estimate the cellular fluorescent intensity to determine the levels of p16 and p15 proteins. Results :Negative expression for p16 protein was found in 10 of 23 samples,and 8 of 23 were p15 negative expression.Both kinds of proteins were abscent in 6 samples.2 of 3 cases of T ALL were negative expression of p16,p15 protein.In non T ALL,6 of 13 were negative expression for p16 protein,5 of 13 were p15 protein deficient.The expression rates of p16,p15 protein in high leukocyte group were lower than those of non high leukocyte group(P<0.05).The expression rates of p16,p15 proteins in HR ALL were lower than those of SR ALL(P<0.05).Conclusion:The p16 and p15 proteins take part in the genesis of ALL.Negative expression of p16,p15 proteins might imply the poor clinical outcome.

关 键 词：P16蛋白 P15蛋白 急性淋巴细胞白血病 流式细胞仪 

分 类 号：R733.71[医药卫生—肿瘤] R730.231[医药卫生—临床医学]