双向电泳分析普洱茶对小鼠肝脏MUP-1蛋白表达的影响  

Effect of Pu-er-tea on Major Urinary Protein Expression in Mouse Liver

作　　者：宋爽[1,2] 严亮[1,4] 王宣军[2] 郝淑美[3] 盛军[1,2] 

机构地区：[1]吉林大学,生命科学学院,吉林长春130012 [2]云南农业大学,普洱茶学教育部重点实验室,云南昆明650201 [3]云南大学校长办公室,云南昆明650228 [4]普洱茶研究院,云南普洱665000

出　　处：《西南农业学报》2014年第1期428-432,共5页Southwest China Journal of Agricultural Sciences

基　　金：云南省科技创新强省计划(2009AB021);云南省自然科学基金面上项目(2012FB151)

摘　　要：本文采用双向电泳技术,鉴定昆明鼠在饮用普洱茶后肝脏蛋白质组变化.结果表明,采用pH3～10胶条未达到理想的分离效果,在采用窄范围pH4～7胶条电泳后得到16个差异蛋白,其中15个蛋白经质谱鉴定得到蛋白的信息,主要尿蛋白（Major urinary protein,MUP-1）表达显著上调,该蛋白可以作为能量代谢的生物标志物,并在近年来被证明具有调节代谢的作用.饮用普洱茶后小鼠肝脏中MUP-1上调,说明普洱茶在小鼠糖代谢中具有促进作用,这些结果从另一个角度解释过去研究中普洱茶保健作用的机理.In this paper,2D-Electrophoresis was employed to analyze the differential expression of mouse liver proteome.The result showed that slight difference was detected with 3-10 strips,sixteen difference expression proteins were detected with pH 4-7 strips,and 15 protein ID were verified with ESI-MS-MS succeesfully,of which major urinary protein（MUP-1） was one of up regulated protein,which served as the biomarker of energy metabolism,testifying the regulating role of metabolism in recent years.The increasing of MUP-1 expression suggested that Pu-er tea promoted the glucose metabolism in mice liver.The results revealed the function of Pu-er-tea in a novel view points.

关 键 词：双向电泳 普洱茶 主要尿蛋白 葡萄糖代谢 

分 类 号：S285.5[农业科学—农业水土工程]