CD133^＋直肠癌细胞对直肠癌放射线敏感性的影响  

Effect of CD133^ ＋ cells on the radiosensitivity of rectal cancer cells

作　　者：裘建明[1] 杨关根[1] 沈忠[1] 丁磊[1] 王东[1] 王辰[1] 

机构地区：[1]杭州市第三人民医院肛肠外科,310009

出　　处：《中华肿瘤杂志》2014年第2期103-107,共5页Chinese Journal of Oncology

基　　金：浙江省自然科学基金（Y2110782）;浙江省科技厅公益技术研究社会发展项目（2012C33097）;浙江省中医药科学研究基金计划（2013ZB103）;国家自然科学基金青年项目（81101876/H1617）;浙江省医药卫生平台骨干人才计划（2011RCA035）

摘　　要：目的探讨CD133^＋直肠癌细胞对直肠癌放射线敏感性的影响。方法采用免疫磁珠分选法将直肠癌细胞株SW480分为CD133^＋和CD133一细胞，Westernblot检测其CD133蛋白表达，克隆形成实验检测其克隆形成能力，annexinV—PI法检测放射线照射后细胞凋亡情况。分离、培养人原代直肠癌细胞，制备荷瘤裸鼠，免疫组化法检测肿瘤组织中CD133的表达情况，并观察放射线照射后荷瘤裸鼠的肿瘤生长情况。结果克隆形成实验显示，CD133^＋和CD133一直肠癌细胞的克隆形成率分别为（67．5±5．5）％和（5．1±3．3）％，CD133^＋直肠癌细胞的克隆形成能力显著强于CD133^-细胞群（P〈0．01）。经放射线照射后，CD133^＋和CD133^-直肠癌细胞的凋亡率分别为（12．6±3．2）％和（38．8±6．7）％，差异有统计学意义（P〈0．01）。荷瘤裸鼠肿瘤组织照射后第9天，CD133高表达照射组和CD133低表达照射组裸鼠的相对肿瘤体积分别为3．00±0．32和2．55±0．29，差异有统计学意义（P〈0．05）。结论CD133^＋直肠癌细胞群能显著提高直肠癌对放射线的耐受性，是增强直肠癌放疗敏感性的潜在靶点。Objective To investigate the effect of CD133^ ＋ cells on radiosensitivity of rectal cancer cells. Methods In vitro experiments： CD133^ ＋ ceils were purified with Immunomagnetic beads from human rectal cancer cell line SW480 cells and annexin V/PI staining was used to determine apoptosis in CD133^ ＋ and CD133- ceils. In vivo experiments： Transplanted rectal tumor was established in 30 nude mice using primarily established SW480 cells. The tumor cells were divided into CDl$$-high and CDl$3-1ow groups based on the immunohistochemical staining of CD133 expression of the tumor cells. The tumor size after irradiation was recorded every three days. Results CD133^ ＋ cells had a much lower percentage of apoptosis after radiation exposure compared with CD133 - cells [ （ 12.6 ±3.2） % vs. （38.8 ± 6.7 ） %, P 〈 0.01 ]. In vivo experiment showed that the normalized tumor size of CD133-high group （3.00 ±0. 32 ） became significantly larger than that of the CD133-1ow group （2.55 ± 0. 29 ） at the ninth day and this difference lasted until the observation end （ P 〈 0.05 ）. Conclusions CD133^ ＋ cells have a radioresistant effect on rectal cancer cells and may become a potential therapeutic target in the radiotherapy of rectal cancer.

关 键 词：直肠肿瘤 细胞 CD133 放射疗法 放射敏感性 细胞培养技术 小鼠 裸 

分 类 号：R735.37[医药卫生—肿瘤]