nm23-M1基因在小鼠粒单核细胞白血病中的表达  

Expression of nm23-M1 Gene in Mouse Myelomonocytic Leukemia

作　　者：董征[1] 孙雪冬[1] 满秋红[1] 姚波[1] 李玉芳[1] 刘铁强[1] 黄珊[1] 刘广贤[1] 艾辉胜[1] 郭梅[1] 

机构地区：[1]解放军307医院,北京100071

出　　处：《实验动物与比较医学》2014年第1期42-45,共4页Laboratory Animal and Comparative Medicine

摘　　要：目的 探讨nm23-M1基因在小鼠粒单核细胞白血病中的表达.方法 给小鼠经尾静脉注射粒单核细胞白血病细胞株WEHI-3,同时选取正常小鼠作为对照组,取发病小鼠以及正常小鼠的骨髓,采取qRT-PCR的方法检测nm23-M1基因的表达情况,分析其与白血病发生及发展的关系.结果 粒单细胞白血病小鼠骨髓细胞中nm23-M1基因的相对表达量是正常对照组中的24倍,两组之间有显著性差异（P＜0.05）.结论 nm23-M1基因在小鼠粒单核细胞白血病中高表达,并且与外周血白细胞数、骨髓原始细胞数以及脏器白血病侵袭情况相关,而与白血病小鼠的性别、年龄无关.Objective To investigate the expression level of nm23-M1 gene in mouse myelomonocytic leukemia. Methods The mice were inoculated intravenously with myelomonocytic leukemia cells of WEHI-3 ceils, and selected a control group of normal mice. Bone marrow samples were collected in two groups. The nm23-M1 mRNA expression were detected by TaqMan quantitative real-time reverse transcrip- tion polymerase chain reaction（qRT-PCR） and analysis of its relationship with the occurrence and devel- opment of leukemia. Results The expression of nm23-M1 gene in bone marrow cells of mice with leukemia is 24 times of the normal control group. There was a significant difference between the two groups （P〈0.05）. Conclusion The nm23-M1 gene overexpressed .in mouse myelomonocytic leukemia, and had close relationship between and peripheral white blood cell count, the number of original cells in bone marrow and organ against the leukemia cases, regardless of the gender, age and leukemia in mice.

关 键 词：nm23-M1基因 粒单核细胞白血病 

分 类 号：R733.7[医药卫生—肿瘤] R-332[医药卫生—临床医学]