Wnt5a对人肝星状细胞促炎细胞因子IL-1β及IL-6表达的影响  

Wnt5a increases the expression of proinflammatory cytokines IL-1β and IL-6 in human hepatic stel- late cells

作　　者：董爽[1] 吴晨明[1] 胡君伟 王芹芹[1] 简易成[1] 何益[1] 钟杰[1] 熊伍军[1] 

机构地区：[1]同济大学附属东方医院肝脏病科,上海200120

出　　处：《中华微生物学和免疫学杂志》2014年第1期37-41,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（81270516）

摘　　要：目的研究Wnt5a对肝星状细胞（nsc）促炎细胞因子IL-1β及IL-6表达的影响，探讨Wnt5a在肝纤维化中的作用及机制。方法采用脂多糖（LPS）和肿瘤坏死因子（TNF-α）刺激人肝星状细胞株LX-2，以qRT—PCR和Western blot检测Wnt5a表达水平变化；分别利用Wnt5a重组蛋白体外刺激LX-2和Wnt5a shRNA病毒转染LX-2，以qRT-PCR、ELISA检测促炎细胞因子IL-1β、IL-6的水平变化。结果LPS及TNF-β可显著刺激LX-2 Wnt5a表达增加（P〈0．05）；Wnt5a重组蛋白可促进LX-2细胞IL-1β、IL-6表达和分泌（P〈0．05）；Wnt5a shRNA转染LX-2后IL-1β、IL-6表达水平显著降低（P〈0．05），ELISA发现Wnt5ashRNA转染组培养上清IL-1β、IL-6水平显著低于pLVX-NC—shRNA组与LX-2组（P〈0．05）。结论Wnt5a可促进HSC促炎细胞因子IL-1β、IL-6的表达而参与肝纤维化进程。[ Abstract ] Objective To study the effects of Wnt5a on the expression of proinflammatory cyto- kines IL-1β and IL-6 in human hepatic stellate cells and its pathogenic mechanism in liver fibrosis. Meth- ods The expression of WntSa at mRNA and protein levels in LPS-stimulated and TNF-α-stimulated human hepatic stellate cell line LX-2 were analyzed by quantitative real-time PCR and Western blot. LX-2 cells were treated with human recombinant Wnt5a protein and transfected by lentivirus carrying Wnt5a shRNA, respectively. The expression of proinflammatory cytokines IL-1β and IL-6 in treated cells were examined by qRT-PCR and ELISA. Results LPS and TNF-α enhanced the expression of WntSa in LX-2 cells （P〈 0.05）. Recombinant Wnt5a protein significantly increased the expression and secretion of IL-1β and IL-6 （P〈0.05）. Compared with pLVX-NC-shRNA cells and LX-2 cells, the expression and secretion of IL-1β and IL-6 in pLVX-Wnt5a-shRNA transfected LX-2 cells were significantly decreased （P〈0.05）. Conclu- sion WntSa might participate in the progression of hver fibrosis through promoting the expression of proin- flammatory cytokines IL-1β and IL-6 in hepatic stellate cells.

关 键 词：WNT5A 肝星状细胞 IL-1Β IL-6 肝纤维化 

分 类 号：R575[医药卫生—消化系统]