环孢素A致牙龈增生与菌斑、胶原代谢的相关性研究  

Oral Care and Collagen Metabolism in the Development of CIGO

作　　者：赵婧[1] 高明津[1] 姜蕾[1] 赵云富[1] 

机构地区：[1]第二军医大学附属长征医院口腔科,上海200003

出　　处：《口腔颌面外科杂志》2014年第1期10-15,共6页Journal of Oral and Maxillofacial Surgery

基　　金：2011年国家自然基金资助项目(81170966)

摘　　要：目的:探讨菌斑存在与否对环孢素A(CsA)所致牙龈增生(CIGO)的影响及其可能的发病机制。方法 :SD雄性大鼠40只,实验组、对照组各20只,均拔除左侧上颌磨牙,在同一SD大鼠口内建立有/无菌斑条件下的牙龈模型。实验组每天胃饲含CAS的橄榄油溶液(30 mg/kg),对照组胃饲等量纯橄榄油。定期观察两组大鼠牙龈增生情况,运用免疫组化和RT-PCR方法观察两组大鼠牙龈内CyPA、CD147、MMP-2的表达差异。结果:实验组上颌双侧牙龈增生明显,同一大鼠口内拔牙侧和非拔牙侧牙龈增生程度无显著差异;CyPA和MMP-2的蛋白和mRNA表达水平实验组较对照组均明显减少(P<0.01);CD147的蛋白质和mRMA表达水平在两组间均无显著差异(P>0.05);实验组和对照组中有牙侧和无牙侧CyPA、CD147和MMP-2的蛋白质和mRMA表达水平均无显著差异(P>0.05)。结论:即使无菌斑存在,仍有CIGO发生;CyPA与MMP-2表达的下降,可能是牙龈组织中胶原降解减少、牙龈增生的原因之一。Objective： The aim of the study was to investigate the role of the plaque formation on the development of cyelosporine A induced gingival overgrowth （CIGO）. Methods： 40 Sprague-Dawley rats were selected and subjected to all left maxillary molars extraction. All rats were randomly divided into two groups. Rats in the test grouP （n=20） received cyclosporine A （CsA）, which was dissolved in olive oil via gastric feeding （30 mg/kg body weight every day） to establish a rat model of the CIGO. Rats in the control group （n=20） received olive oil only. After 4 weeks, rats were sacrificed and gingival tissues were observed by macroscopic and microscopic methods. The expressions of CyPA, CD147 and MMP-2 in gingival tissues were evaluated by immunohistochemistry and Real-time PCR. Results： In the CsA-treated animals, both sides of the maxillary gingival were found to be enlarged and the body weights reduced. In the control group, expressions of CyPA and MMP-2 at protein and mRNA levels were obviously higher than that in test group（P〈0.01）. But no significant difference was found in CD147 expression between two groups （P〉0.05）. Conclusion： Oral care manifested as plaque formation is the relevant factor but not initiating factor of CIGO. The mechanism of CIGO may be concerned with the formation of CsA-CyPA complex and competitive inhibition of the interaction between CyPA and CD147, leading to imbalance of collagen degradation and accumulation, which causes gingival overgrowth.

关 键 词：环孢素A 牙龈增生 菌斑 基质金属蛋白酶-2 CD147 亲环素A 

分 类 号：R781.4[医药卫生—口腔医学]